植物资源与环境学报
植物資源與環境學報
식물자원여배경학보
JOURNAL OF PLANT RESOURCES AND ENVIRONMENT
2015年
1期
28-35
,共8页
王丰青%田云鹤%黄勇%李娟%董诚明%张重义
王豐青%田雲鶴%黃勇%李娟%董誠明%張重義
왕봉청%전운학%황용%리연%동성명%장중의
地黄%种质%表型性状%聚类分析%主成分分析%相关性分析
地黃%種質%錶型性狀%聚類分析%主成分分析%相關性分析
지황%충질%표형성상%취류분석%주성분분석%상관성분석
Rehmannia glutinosa( Gaertn.) Libosch. ex Fisch. et C. A. Mey.%germplasm%phenotypic character%cluster analysis%principal component analysis%correlation analysis
对地黄[Rehmannia glutinosa (Gaertn.) Libosch. ex Fisch. et C. A. Mey.]11个主栽品种、3个变异类型和4个野生种源的18个表型性状(包括9个数值型性状和9个非数值型性状)进行了观测和分析;在此基础上,对18个地黄种质进行了聚类分析,还进行了18个表型性状的主成分分析和9个数量性状的相关性分析。结果表明:18个地黄种质的表型性状变异非常丰富,冠幅、茎粗、单株叶片数、叶长、叶宽、单株块根数、块根长度、块根直径和单株块根鲜质量变异均较大,其变异系数大多在20%左右,其中单株块根鲜质量的变异系数(56.66%)最大。聚类分析结果显示:在欧氏距离6.74处,18个地黄种质可分为栽培、野生和新变异类型3大类。主成分分析结果表明:在18个表型性状中,前4个主成分的累计贡献率达81.8655%,其中第1主成分的贡献率为50.5527%,包括冠幅、叶长、叶宽、块根直径和单株块根鲜质量。相关性分析结果显示:单株块根鲜质量与冠幅、茎粗、单株叶片数、叶长、叶宽和块根直径呈极显著正相关(P<0.01),相关系数分别为0.862、0.849、0.759、0.879、0.822和0.898;与块根长度呈显著正相关(P<0.05),相关系数为0.562。研究结果显示:地黄种质间存在较大的表型变异,基于表型性状的聚类分析结果可用于鉴别地黄种质间的亲缘关系;并可以通过冠幅、茎粗、叶片大小和块根大小等性状的选择提高地黄的单株块根产量。
對地黃[Rehmannia glutinosa (Gaertn.) Libosch. ex Fisch. et C. A. Mey.]11箇主栽品種、3箇變異類型和4箇野生種源的18箇錶型性狀(包括9箇數值型性狀和9箇非數值型性狀)進行瞭觀測和分析;在此基礎上,對18箇地黃種質進行瞭聚類分析,還進行瞭18箇錶型性狀的主成分分析和9箇數量性狀的相關性分析。結果錶明:18箇地黃種質的錶型性狀變異非常豐富,冠幅、莖粗、單株葉片數、葉長、葉寬、單株塊根數、塊根長度、塊根直徑和單株塊根鮮質量變異均較大,其變異繫數大多在20%左右,其中單株塊根鮮質量的變異繫數(56.66%)最大。聚類分析結果顯示:在歐氏距離6.74處,18箇地黃種質可分為栽培、野生和新變異類型3大類。主成分分析結果錶明:在18箇錶型性狀中,前4箇主成分的纍計貢獻率達81.8655%,其中第1主成分的貢獻率為50.5527%,包括冠幅、葉長、葉寬、塊根直徑和單株塊根鮮質量。相關性分析結果顯示:單株塊根鮮質量與冠幅、莖粗、單株葉片數、葉長、葉寬和塊根直徑呈極顯著正相關(P<0.01),相關繫數分彆為0.862、0.849、0.759、0.879、0.822和0.898;與塊根長度呈顯著正相關(P<0.05),相關繫數為0.562。研究結果顯示:地黃種質間存在較大的錶型變異,基于錶型性狀的聚類分析結果可用于鑒彆地黃種質間的親緣關繫;併可以通過冠幅、莖粗、葉片大小和塊根大小等性狀的選擇提高地黃的單株塊根產量。
대지황[Rehmannia glutinosa (Gaertn.) Libosch. ex Fisch. et C. A. Mey.]11개주재품충、3개변이류형화4개야생충원적18개표형성상(포괄9개수치형성상화9개비수치형성상)진행료관측화분석;재차기출상,대18개지황충질진행료취류분석,환진행료18개표형성상적주성분분석화9개수량성상적상관성분석。결과표명:18개지황충질적표형성상변이비상봉부,관폭、경조、단주협편수、협장、협관、단주괴근수、괴근장도、괴근직경화단주괴근선질량변이균교대,기변이계수대다재20%좌우,기중단주괴근선질량적변이계수(56.66%)최대。취류분석결과현시:재구씨거리6.74처,18개지황충질가분위재배、야생화신변이류형3대류。주성분분석결과표명:재18개표형성상중,전4개주성분적루계공헌솔체81.8655%,기중제1주성분적공헌솔위50.5527%,포괄관폭、협장、협관、괴근직경화단주괴근선질량。상관성분석결과현시:단주괴근선질량여관폭、경조、단주협편수、협장、협관화괴근직경정겁현저정상관(P<0.01),상관계수분별위0.862、0.849、0.759、0.879、0.822화0.898;여괴근장도정현저정상관(P<0.05),상관계수위0.562。연구결과현시:지황충질간존재교대적표형변이,기우표형성상적취류분석결과가용우감별지황충질간적친연관계;병가이통과관폭、경조、협편대소화괴근대소등성상적선택제고지황적단주괴근산량。
Eighteen phenotypic characters ( including nine numeric characters and nine non numeric characters) of eleven main cultivars, three mutations and four wild provenances of Rehmannia glutinosa ( Gaertn.) Libosch. ex Fisch. et C. A. Mey. were observed and analyzed. On this basis, cluster analysis on eighteen germplasms of R. glutinosa was carried out, and principal component analysis on eighteen phenotypic characters and correlation analysis on nine quantitative characters were also done. The results show that variations of phenotypic characters of eighteen germplasms of R. glutinosa are very rich. Variations of crown width, stem diameter, leaf number per plant, leaf length, leaf width, tuberous root number per plant, tuberous root length, tuberous root diameter and tuberous root fresh weight per plant all are bigger, with coefficients of variation about 20% in most cases, in which, coefficient of variation (56. 66%) of tuberous root fresh weight per plant is the biggest. The cluster analysis result shows that at Euclidean distance of 6. 74, eighteen germplasms of R. glutinosa are divided into three groups of cultivation, wild and new variation types of R. glutinosa. The principal component analysis result shows that in eighteen phenotypic characters, the accumulative contribution rate of the first four principal components reaches to 81. 865 5%, in which, the contribution rate of the first principal component is 50. 552 7%, which includes crown width, leaf length, leaf width, tuberous root diameter and tuberous root fresh weight per plant. The correlation analysis result shows that tuberous root fresh weight per plant has extremely significantly positive correlation ( P<0. 01 ) with crown width, stem diameter, leaf number per plant, leaf length, leaf width and tuberous root diameter, their correlation coefficients are 0. 862, 0. 849, 0. 759, 0. 879, 0. 822 and 0. 898, respectively, and has significantly positive correlation ( P<0 . 05 ) with tuberous root length with the correlation coefficient of 0 . 562 . It is suggested that there is larger phenotypic variation among germplasms of R. glutinosa. The cluster analysis result based on phenotypic characters can be used for identifying genetic relationship of R. glutinosa germplasms. And the tuberous root yield per plant of R. glutinosa can be improved by selection of crown width, stem diameter, leaf size and tuberous root size, etc.