昆明理工大学学报（自然科学版）
昆明理工大學學報（自然科學版）
곤명리공대학학보（자연과학판）
JOURNAL OF KUNMING UNIVERSITY OF SCIENCE AND TECHNOLOGY（SCIENCE AND TECHNOLOGY）
2015年
2期
88-95
,共8页
微藻%油脂产率%中心组合试验%响应面法%异养培养
微藻%油脂產率%中心組閤試驗%響應麵法%異養培養
미조%유지산솔%중심조합시험%향응면법%이양배양
microalgae%lipid productivity%central composite design%response surface methodology%hetero-trophic cultivation
为了提高微藻 Chlorella sp．U4341的油脂产率，采用响应面法对异养培养条件进行优化研究．通过单因素试验筛选出促进 Chlorella sp．U4341油脂产率的最佳碳源、氮源分别为葡萄糖、NaNO3．利用 Plackett －Burman 试验筛选出显著影响 Chlorella sp．U4341油脂产率的三个因子，通过最陡爬坡试验逼近最大油脂产率区域，运用中心组合试验及响应面分析确定了三个因子的最佳浓度：葡萄糖为24.81 g／L，NaNO3为1.68 g／L，K2 HPO4·3H2 O 为0.58 g／L．优化后的条件培养微藻 Chlorella sp．U4341的油脂产率为273.34 mg／L·d，比优化前提高了58％．
為瞭提高微藻 Chlorella sp．U4341的油脂產率，採用響應麵法對異養培養條件進行優化研究．通過單因素試驗篩選齣促進 Chlorella sp．U4341油脂產率的最佳碳源、氮源分彆為葡萄糖、NaNO3．利用 Plackett －Burman 試驗篩選齣顯著影響 Chlorella sp．U4341油脂產率的三箇因子，通過最陡爬坡試驗逼近最大油脂產率區域，運用中心組閤試驗及響應麵分析確定瞭三箇因子的最佳濃度：葡萄糖為24.81 g／L，NaNO3為1.68 g／L，K2 HPO4·3H2 O 為0.58 g／L．優化後的條件培養微藻 Chlorella sp．U4341的油脂產率為273.34 mg／L·d，比優化前提高瞭58％．
위료제고미조 Chlorella sp．U4341적유지산솔，채용향응면법대이양배양조건진행우화연구．통과단인소시험사선출촉진 Chlorella sp．U4341유지산솔적최가탄원、담원분별위포도당、NaNO3．이용 Plackett －Burman 시험사선출현저영향 Chlorella sp．U4341유지산솔적삼개인자，통과최두파파시험핍근최대유지산솔구역，운용중심조합시험급향응면분석학정료삼개인자적최가농도：포도당위24.81 g／L，NaNO3위1.68 g／L，K2 HPO4·3H2 O 위0.58 g／L．우화후적조건배양미조 Chlorella sp．U4341적유지산솔위273.34 mg／L·d，비우화전제고료58％．
To increase the lipid productivity of microalgae Chlorella sp.U4341 ,optimization of heterotrophic cul-tivation condition is carried out by response surface method.The optimal carbon and nitrogen sources for Chlorella sp.U4341 are glucose and sodium nitrate,respectively.Three most important influencing factors for lipid produc-tivity of Chlorella sp.U4341 are screened via Plackett -Burman experiment.Subsequently,the steepest ascent ex-periment is used to guide key factors to approach the maximum lipid productivity region.Optimal concentrations of the three influencing factors are confirmed by central composite design and response surface analysis.The re-sults are 24.81 g/L of glucose,1 .68 g/L of sodium nitrate and 0.58 g/L of dipotassium phosphate trihydrate. Cultivated with the optimized condition,the lipid productivity of Chlorella sp.U4341 reaches 273.34 mg/L·d, increased by 58% compared with the original cultivation condition.
