CAJ | 학술논문

以淡水发光菌———青海弧菌（Vibrio qinghaiensis sp.-Q67）为检测生物，应用VeritasTM微板光度计分别测定了全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）对Q67的毒性效应。结果表明：全氟辛酸为典型的S型剂量－效应关系，利用Weibull非线性函数进行曲线模拟，模拟函数可以有效估计化合物的半抑制毒性效应值EC50。全氟辛烷磺酸对Q67的毒性为非单调的U 型剂量－效应关系，运用最优权重理论和三次曲线回归方法，通过SPSS统计软件对剂量－效应曲线进行拟合，得到其三次回归曲线方程。结果表明：在浓度介于（5.031E－4）～（7.133E－3）mol／L ，全氟辛烷磺酸对Q67毒性效应为自稳态区域，表现为Q67的发光强度增强，即在低浓度作用条件下，PFOA对Q67的毒性表现为抑制作用，而PFOS则表现为刺激兴奋作用。
이담수발광균———청해호균（Vibrio qinghaiensis sp.-Q67）위검측생물，응용VeritasTM미판광도계분별측정료전불신산（PFOA）화전불신완광산（PFOS）대Q67적독성효응。결과표명：전불신산위전형적S형제량－효응관계，이용Weibull비선성함수진행곡선모의，모의함수가이유효고계화합물적반억제독성효응치EC50。전불신완광산대Q67적독성위비단조적U 형제량－효응관계，운용최우권중이론화삼차곡선회귀방법，통과SPSS통계연건대제량－효응곡선진행의합，득도기삼차회귀곡선방정。결과표명：재농도개우（5.031E－4）～（7.133E－3）mol／L ，전불신완광산대Q67독성효응위자은태구역，표현위Q67적발광강도증강，즉재저농도작용조건하，PFOA대Q67적독성표현위억제작용，이PFOS칙표현위자격흥강작용。
In freshwater luminescent bacteria (Vibrioqinghaiensis sp.-Q67)for biological detection,the inhibi-tion toxicity of perfluorinated octylic acid (PFOA)and perfluorinated octane sulfonic acid (PFOS)to Q67 was tested by VeritasTM photometer.In Weibull nonlinear function curve simulation,the dose-response relationship of PFOA in Q67 was typical S-type curve.The simulation function can effectively estimate semi-inhibition effect value (EC50 )of compounds.The inhibition curve of perfluorooctane sulfonate in Q67 was non-monotonic U-type curve.The dose-response curve was finished by the optimal weight curve theory and three regression curve e-quation with SPSS statistical software.The results show that the toxicity effect of PFOS on the Q67 has a low-dose stimulating effect when the concentration was between 5.031E-4 mol/L and 7.133E-3 mol/L.There is homeostasis region,and the luminescence intensity of Q67 is enhanced.The study shows that under the condi-tion of low concentration effect,PFOA toxicity of Q67 characterizes inhibition,whereas PFOS performs stimula-tive effect.