国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2015年
5期
777-780
,共4页
补肾养血明目方%ARPE-19 细胞%丙烯醛%氧化应激
補腎養血明目方%ARPE-19 細胞%丙烯醛%氧化應激
보신양혈명목방%ARPE-19 세포%병희철%양화응격
the prescription of reinforcing kidney, %nourishing blood,improving eyesight%ARPE- 19 cell%acrolein%oxidative stress
目的：利用丙烯醛建立ARPE-19细胞氧化应激模型,观察补肾养血明目方含药血清对丙烯醛诱导ARPE-19细胞氧化损伤的保护作用。<br> 方法：制备SD 大鼠补肾养血明目方含药血清及空白血清,通过CCK-8检测不同浓度(2.5%,5%,10%,20%,40%)血清对细胞活性的影响,预先加入1.25%,2.5%,5%等不同浓度的含药血清处理24 h 后,将终浓度为75μmol/L的丙烯醛加入对数生长期的ARPE-19细胞中处理24h, CCK-8法检测含药血清对细胞活力的影响, DAPI染色观察细胞核形态。<br> 结果：CCK-8结果示：低浓度(2.5%,5%)血清对细胞活性影响不大( P>0.05),高浓度(10%,20%,40%)血清降低细胞活性(P<0.05),补肾养血明目方含药血清中、高剂量组细胞活性较空白血清组有统计学意义(P<0.05)；<br> 结论：补肾养血明目方对丙烯醛诱导的ARPE-19细胞损伤有保护作用。
目的：利用丙烯醛建立ARPE-19細胞氧化應激模型,觀察補腎養血明目方含藥血清對丙烯醛誘導ARPE-19細胞氧化損傷的保護作用。<br> 方法：製備SD 大鼠補腎養血明目方含藥血清及空白血清,通過CCK-8檢測不同濃度(2.5%,5%,10%,20%,40%)血清對細胞活性的影響,預先加入1.25%,2.5%,5%等不同濃度的含藥血清處理24 h 後,將終濃度為75μmol/L的丙烯醛加入對數生長期的ARPE-19細胞中處理24h, CCK-8法檢測含藥血清對細胞活力的影響, DAPI染色觀察細胞覈形態。<br> 結果：CCK-8結果示：低濃度(2.5%,5%)血清對細胞活性影響不大( P>0.05),高濃度(10%,20%,40%)血清降低細胞活性(P<0.05),補腎養血明目方含藥血清中、高劑量組細胞活性較空白血清組有統計學意義(P<0.05)；<br> 結論：補腎養血明目方對丙烯醛誘導的ARPE-19細胞損傷有保護作用。
목적：이용병희철건립ARPE-19세포양화응격모형,관찰보신양혈명목방함약혈청대병희철유도ARPE-19세포양화손상적보호작용。<br> 방법：제비SD 대서보신양혈명목방함약혈청급공백혈청,통과CCK-8검측불동농도(2.5%,5%,10%,20%,40%)혈청대세포활성적영향,예선가입1.25%,2.5%,5%등불동농도적함약혈청처리24 h 후,장종농도위75μmol/L적병희철가입대수생장기적ARPE-19세포중처리24h, CCK-8법검측함약혈청대세포활력적영향, DAPI염색관찰세포핵형태。<br> 결과：CCK-8결과시：저농도(2.5%,5%)혈청대세포활성영향불대( P>0.05),고농도(10%,20%,40%)혈청강저세포활성(P<0.05),보신양혈명목방함약혈청중、고제량조세포활성교공백혈청조유통계학의의(P<0.05)；<br> 결론：보신양혈명목방대병희철유도적ARPE-19세포손상유보호작용。
?AlM: To explore the effects of the prescription of reinforcing kidney, nourishing blood, improving eyesight on the oxidative stress model of ARPE-19 cells induced by acrolein. <br> ?METHODS:SD rats serum containing the prescription of reinforcing kidney, nourishing blood, improving eyesight and the content of distilled water in serum were prepared. The effects of the prescription and distilled water in serum at different concentration ( 2. 5%, 5%, 10%, 20% and 40%) on cell vitality was observed by cell counting kit ( CCK-8 ) assay. the logarithmic phase of ARPE-19 cells were pretreated by different concentrations (1. 25%, 2. 5%and 5%) of the prescription serum and distilled water in serum for 24h. Then it was treated with 75μmol/L acrolein for 24h. Cell vitality was observed by CCK-8 assay. The change of cell nucleus was detected by DAPl staining . <br> ?RESULTS: 2. 5% and 5% serum had no effect on cell viability (P>0. 05), while 10%, 20%, 40% serum could inhibit cell viability (P<0. 05). CCK-8 results showed that 2. 5% and 5% the prescription of reinforcing kidney, nourishing blood, improving eyesight serum was better than distilled water in serum (P<0. 05). <br> ?CONCLUSlON: The prescription of reinforcing kidney, nourishing blood, improving eyesight has the protective effect on ARPE-19 cell damage induced by acrolein.
