CAJ | 학술논문

目的：探讨维药异常黑胆质成熟剂总黄酮（ASMq）对人慢性骨髓性白血病细胞（K562）及人白血病阿霉素耐药株（K562／ADR）的细胞毒性作用。方法对 K562细胞株及 K562／ADR 细胞株细胞进行培养，在培养过程中注意 K562／ADR 细胞株对阿霉素的耐药性。取对数生长期 K562、K562／ADR 细胞株，96板孔中加入RPMI-1640培养液配制5×104个／mL 细胞悬液100μL／每孔，37℃、5％ CO2培养24 h 贴壁，然后加入不同浓度（0、50、100、200、400、800和1600μg／mL）的 ASMq，在37℃共培养48 h，每组5个重复，按照 CCK-8说明书检测细胞活性。结果K562细胞株对不同浓度的 ASMq 的细胞毒性的敏感性不同，差异有统计学意义（P ＜0．05）；K562／ADR 细胞株对0～1600μg／mL 浓度的 ASMq 的细胞毒性的敏感性差异无统计学意义（P ＞0．05）；结论ASMq 对 K562细胞具有细胞毒性作用，为临床治疗血液肿瘤提供了新的线索。
목적：탐토유약이상흑담질성숙제총황동（ASMq）대인만성골수성백혈병세포（K562）급인백혈병아매소내약주（K562／ADR）적세포독성작용。방법대 K562세포주급 K562／ADR 세포주세포진행배양，재배양과정중주의 K562／ADR 세포주대아매소적내약성。취대수생장기 K562、K562／ADR 세포주，96판공중가입RPMI-1640배양액배제5×104개／mL 세포현액100μL／매공，37℃、5％ CO2배양24 h 첩벽，연후가입불동농도（0、50、100、200、400、800화1600μg／mL）적 ASMq，재37℃공배양48 h，매조5개중복，안조 CCK-8설명서검측세포활성。결과K562세포주대불동농도적 ASMq 적세포독성적민감성불동，차이유통계학의의（P ＜0．05）；K562／ADR 세포주대0～1600μg／mL 농도적 ASMq 적세포독성적민감성차이무통계학의의（P ＞0．05）；결론ASMq 대 K562세포구유세포독성작용，위림상치료혈액종류제공료신적선색。
Objective To analyse cytotoxicity function of the abnormal savda munziq total flavonoids (ASMq)on K562 and K562/ADR of human being.Methods K562 and K562/ADR cells were developed to pay attention to adriamycin resistance of K562/ADR.The logarithmic phase K562 and K562/ADR cells 96-well plates with RPMI-1640 culture make up 5×10 4/mL cells suspension 100 μL/per hole,37℃ and 5%CO2 24 h post wall were pouring into different concentrations (0, 50, 100, 200, 400,800 and 1 600 μg/mL)of 37℃ ASMq trained 48 h to detect cell activity by the instruction (CCK-8).Result There is a statistically significant difference (P <0.05)of K562 with different concentration of ASMq cytotoxic sensitivity.The cytotoxicity of different concentration ASMq to K562/ADR has no statistical significance (P >0.05).Conclusion ASMq has cytotoxic effect on the K562 cells,Blood tumor chemotherapy to re-duce drug resistance for clinical treatment provides a new clue.