CAJ | 학술논문

目的：建立测定天香丹颗粒中红景天苷和丹参酮ⅡA 含量的高效液相色谱法。方法色谱条件：色谱柱为 Hypersil ODS2（4．6 mm×250 mm，5μm）；乙腈-0．03％磷酸水溶液（7：93）作为流动相；检测波长220 nm；流速为1．0 mL／min。结果红景天苷在5．44～16．32μg 范围内与峰面积呈良好的线性关系（r ＝0．9986）；丹参酮ⅡA 在0．074～0．222μg 范围内与峰面积呈良好的线性关系（r ＝0．9995）。红景天苷平均回收率为101．45％， RSD 为1．78％（n ＝6）；丹参酮ⅡA 平均回收率为101．78％，RSD 为1．71％（n ＝6）。天香丹颗粒中红景天苷含量为1．57 mg／g，丹参酮ⅡA 含量为0．58 mg／g。结论本法快捷、简便，结果准确、可靠、重复性好，可作为天香丹颗粒中红景天苷和丹参酮ⅡA 的测定方法。
목적：건립측정천향단과립중홍경천감화단삼동ⅡA 함량적고효액상색보법。방법색보조건：색보주위 Hypersil ODS2（4．6 mm×250 mm，5μm）；을정-0．03％린산수용액（7：93）작위류동상；검측파장220 nm；류속위1．0 mL／min。결과홍경천감재5．44～16．32μg 범위내여봉면적정량호적선성관계（r ＝0．9986）；단삼동ⅡA 재0．074～0．222μg 범위내여봉면적정량호적선성관계（r ＝0．9995）。홍경천감평균회수솔위101．45％， RSD 위1．78％（n ＝6）；단삼동ⅡA 평균회수솔위101．78％，RSD 위1．71％（n ＝6）。천향단과립중홍경천감함량위1．57 mg／g，단삼동ⅡA 함량위0．58 mg／g。결론본법쾌첩、간편，결과준학、가고、중복성호，가작위천향단과립중홍경천감화단삼동ⅡA 적측정방법。
Objective To establish a HPLC method for determination on salidroside and tanshinone Ⅱ A in Tianxiangdan granule.Methods The HPLC system consisted of chromatographic column of Hypersil ODS2 (4.6 mm×250 mm,5 μm);acetonitrile -0.03% phosphoric acid solution (7∶93)as mobile phase;the 220 nm detection wavelength;and 1 mL/min flow rate.Results Salidroside in the range of 5.44 -16.32 μg and the peak area had a good linear relationship (r =0.9986);Tanshinone Ⅱ A in the range of 0.074-0.222 μg and the peak area had a good linear relationship (r =0.9995).The average recovery rate of Salidroside was 101.45% and RSD was 1.78% (n =6);the average recovery rate of Tanshinone Ⅱ A was 101.78% and RSD was 1.71% (n =6).The content of Salidroside was 1.57 mg/g and Tanshinone Ⅱ A was 0.58 mg/g in Tianxiangdan granule.Conclusion This method,quick,simple,accurate,reliable,with good reproducibility,can be used as a method for the determination on salidroside and tanshinone Ⅱ A in Tianxiangdan granule .