CAJ | 학술논문

目的：观察康脑液预处理后对大鼠局灶性脑缺血再灌注脑组织CA1区神经再生的影响。方法将150只健康雄性 Sprague-Dawley 大鼠随机分为康脑液高、中、低3个剂量组（24，12，6 g? kg-1? d-1）、假手术组及模型组，建立右侧局灶性脑缺血模型，于缺血后2 h及24 h进行神经功能缺损评分。于再灌注1，3，7，14 d后，通过HE染色观察海马CA1区细胞的形态学改变，免疫组化检测各组CA1区胶质纤维酸性蛋白（GFAP）和巢蛋白（Nestin）的表达。结果康脑液预处理组在术后的神经功能缺损评分明显降低，能显著抑制CA1区GFAP的表达（ P＜0.01），并显著增强CA1区Nestin的表达（ P＜0.01）。结论康脑液抗脑缺血再灌注损伤可能与促进海马区神经再生及分化、抑制纤维瘢痕形成有关。
목적：관찰강뇌액예처리후대대서국조성뇌결혈재관주뇌조직CA1구신경재생적영향。방법장150지건강웅성 Sprague-Dawley 대서수궤분위강뇌액고、중、저3개제량조（24，12，6 g? kg-1? d-1）、가수술조급모형조，건립우측국조성뇌결혈모형，우결혈후2 h급24 h진행신경공능결손평분。우재관주1，3，7，14 d후，통과HE염색관찰해마CA1구세포적형태학개변，면역조화검측각조CA1구효질섬유산성단백（GFAP）화소단백（Nestin）적표체。결과강뇌액예처리조재술후적신경공능결손평분명현강저，능현저억제CA1구GFAP적표체（ P＜0.01），병현저증강CA1구Nestin적표체（ P＜0.01）。결론강뇌액항뇌결혈재관주손상가능여촉진해마구신경재생급분화、억제섬유반흔형성유관。
Objective To explore the influence of Kangnaoye recipe preconditioning on the nerve regeneration in rats CA 1 area after cerebral ischemia and reperfusion .Methods The 150 rats were randomly divi-ded into sham -operated group , model group , high -dose Kangnaoye group, middle-dose Kangnaoye group and low -dose Kangnaoye group (24, 12, 6 g? kg -1? d-1 ).The modle of cerebral ischemia and reper-fusion ( I/R) was induced by middle cerebral artery occlusion ( MCAO) and recanalization.Neurological symptom score were evaluated .HE stai-ning of the cells was used to observe the morphology changes in hippo-campus CA1 area.Glial ibrillary acidic protein ( GFAP ) protein and Nestin protein were observed by immunohistochemical method in CA 1 area.Results Compared with model group , neurological symptom score of Kangnaoye recipe preconditioning group were decreased and the result was significant ( P<0.01 ) .The groups pretreated with Kangnaoye had a lower expression of GFAP than the model group ( P<0.01 ) and the expression of the Nestin of groups pretreated with Kangnaoye were signifi-cantly higher than the model group (P<0.01) at the time of the 1,3,7 and 14 d after cerebral ischemia.Conclusion The Kangnaoye recipe preconditioning had protective effects on the pathological morphological changing ,it may be associated with promoting the neural stem cells regenera-tion and differentiation as well as inhibition the formation of fibrous scar .