CAJ | 학술논문

目的：观察人尿激肽原酶对脑缺血再灌注损伤模型大鼠脑组织中脑源性神经营养因子表达的影响。方法将SD大鼠随机分为假手术组、模型组和实验组，每组8只。实验组于再灌注后5 min静脉注射人尿激肽原酶3.5×10-3 PNA U? kg -1；假手术组和模型组正常喂养。模型组和实验组用线栓法制作大脑中动脉脑缺血再灌注模型，假手术组大鼠接受相似手术处理，但是不插入线栓。在缺血2 h再灌注48 h后，断头取脑，用半定量聚合酶链式反应法、免疫组织化学法和Western blot 法检测脑组织中脑源性神经营养因子的表达水平。结果与假手术组相比，模型组脑组织中脑源性神经营养因子的表达明显增高（ P＜0.05）；与模型组相比，实验组脑组织中脑源性神经营养因子的表达明显增高（ P＜0.05）。结论人尿激肽原酶可增加脑缺血再灌注损伤后脑组织中脑源性神经营养因子的表达，对脑缺血再灌注损伤起保护作用。
목적：관찰인뇨격태원매대뇌결혈재관주손상모형대서뇌조직중뇌원성신경영양인자표체적영향。방법장SD대서수궤분위가수술조、모형조화실험조，매조8지。실험조우재관주후5 min정맥주사인뇨격태원매3.5×10-3 PNA U? kg -1；가수술조화모형조정상위양。모형조화실험조용선전법제작대뇌중동맥뇌결혈재관주모형，가수술조대서접수상사수술처리，단시불삽입선전。재결혈2 h재관주48 h후，단두취뇌，용반정량취합매련식반응법、면역조직화학법화Western blot 법검측뇌조직중뇌원성신경영양인자적표체수평。결과여가수술조상비，모형조뇌조직중뇌원성신경영양인자적표체명현증고（ P＜0.05）；여모형조상비，실험조뇌조직중뇌원성신경영양인자적표체명현증고（ P＜0.05）。결론인뇨격태원매가증가뇌결혈재관주손상후뇌조직중뇌원성신경영양인자적표체，대뇌결혈재관주손상기보호작용。
Objective To observe the effects of urinary kallidinogenase on the expression of brain derived neurotrophic factor ( BDNF ) in rats with focal cerebral ischemia -reperfusion.Methods SD rats were ran-domly assigned into three groups:sham operation group ( sham group ) , focal cerebral ischemia -reperfusion group ( model group ) and urinary kallidinogenase group ( test group ) , with eight in each group .The middle cerebral artery occlusion reperfusion model was made by the suture method(ischemia for 2 hours, and reperfusion for 48 hours).The rats in the test group were injected with urinary kallidinogenase 3.5 ×10 -3 PNA U? kg -1 after making a model.The level of BDNF expression were measured by PCR , immunohistochemistry and Western blot . Results Compared with sham group , the expression of BDNF was in-creased in model group(P<0.05).Compared with model group, the ex-pression of BDNF was increased in test group ( P<0.05 ) .Conclusion Urinary kallidinogenase has a protective effect on ischemic brain injury by increasing the BDNF expression in ischemic brain .