CAJ | 학술논문

目的：探讨唾液酸转移酶（ST6Gal I）对人胃癌细胞（SGC7901）增殖和迁移能力的影响。方法：采用脂质体2000（Lipofectamine 2000），构建ST6Gal I高表达和ST6Gal I低表达细胞株，以空载体（Vector）作为对照组。采用实时荧光定量PCR（real-time PCR，RT-PCR）检测转染后SGC7901细胞中ST6Gal I的mRNA水平；用细胞CCK-8试剂盒及Transwell小室实验检测转染前后细胞的增殖、迁移情况。结果：成功构建稳定转染单克隆细胞株分别为Vector、X61-4和PS7；与Vector组相比，PS7组的mRNA表达水平显著增加（P＜0.05），X61-4组则显著降低（P＜0.05）；X61-4的细胞增殖明显高于PS7细胞（P＜0.05）；胃癌细胞ST6Gal I的表达水平与细胞迁移能力成正相关，PS7组的迁移率显著高于X61-4组（P＜0.01）和Vector组（P＜0.01）， X61-4组的迁移率低于Vector组（P＜0.05）。结论：细胞实验表明，降低ST6Gal I的表达可以有效促进胃癌SGC7901细胞的增殖能力，而上调ST6Gal I可以增强胃癌细胞的迁移能力。因此，ST6Gal I是与肿瘤增殖和转移相关的基因，是潜在的肿瘤分子治疗靶点。
목적：탐토타액산전이매（ST6Gal I）대인위암세포（SGC7901）증식화천이능력적영향。방법：채용지질체2000（Lipofectamine 2000），구건ST6Gal I고표체화ST6Gal I저표체세포주，이공재체（Vector）작위대조조。채용실시형광정량PCR（real-time PCR，RT-PCR）검측전염후SGC7901세포중ST6Gal I적mRNA수평；용세포CCK-8시제합급Transwell소실실험검측전염전후세포적증식、천이정황。결과：성공구건은정전염단극륭세포주분별위Vector、X61-4화PS7；여Vector조상비，PS7조적mRNA표체수평현저증가（P＜0.05），X61-4조칙현저강저（P＜0.05）；X61-4적세포증식명현고우PS7세포（P＜0.05）；위암세포ST6Gal I적표체수평여세포천이능력성정상관，PS7조적천이솔현저고우X61-4조（P＜0.01）화Vector조（P＜0.01）， X61-4조적천이솔저우Vector조（P＜0.05）。결론：세포실험표명，강저ST6Gal I적표체가이유효촉진위암SGC7901세포적증식능력，이상조ST6Gal I가이증강위암세포적천이능력。인차，ST6Gal I시여종류증식화전이상관적기인，시잠재적종류분자치료파점。
Objective: To explore the effect of α2, 6 sialytransferase (ST6Gal I) on the abilities of proliferation and migration in SGC7901 cells. Methods: Gastric cancer cell lines SGC7901 were con-structed to ST6Gal I high expression (PS7) and ST6Gal I lower expression (X61) with lipofectamine 2000 and empty vector (Vector) was used as a control group. The expression of ST6Gal I was examined with RT-PCR. Cell proliferation was evaluated with the CCK-8 kit, and migration was evaluated with Transwell chamber assay. Results: The 3 stable transfected cell lines were constructed successfully. The most effec-tive clone cells were chosed as our experimental cells, and named as Vector, X61-4 and PS7. In contrasted with Vector, the expression of ST6GAL I in X61-4 was statistically significant lower (P<0.05), and PS7 was statistically significant higher (P<0.05). Cell growth curve showed that cell proliferation of X61-4 was obviously higher than that of PS7 cells (P<0.05). The transwell chamber assay results showed that the migra-tion of PS7 was signiifcantly higher than that of the X61-4 (P<0.01) and Vector (P<0.01), X61-4 was lower than that of the Vector (P<0.05). Conclusion:Cell experiment results show that lower ST6Gal I can effectively pro-mote the proliferation ability of SGC7901 gastric cancer cells, but high ST6Gal I can enhance gastric cancer cell migration ability. Therefore, ST6Gal I is related to the tumor proliferation and metastasis, and it is also a potential tumor molecular therapeutic targets.