中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2015年
4期
237-239
,共3页
银屑病%miR-203%基因,p63%生存素
銀屑病%miR-203%基因,p63%生存素
은설병%miR-203%기인,p63%생존소
Psoriasis%MiR-203%Genes,p63%Survivin
目的 检测寻常性银屑病皮损中miR-203及其下游靶基因p63、生存素的表达水平.方法 实时PCR法检测30例寻常性银屑病患者皮损及30例健康对照皮肤中miR-203、p63、生存素mRNA的表达水平,miR-203以U6为内参照,p63和生存素以GAPDH为内参照;Western印迹法检测靶基因p63、生存素蛋白表达水平,两组间比较采用t检验,寻常性银屑病皮损中miR-203与生存素、p63表达的相关性采用Pearson相关分析.结果 与健康对照皮肤相比,寻常性银屑病皮损中miR-203 mRNA表达量为对照组的(0.41±0.11)倍,表达明显下调(t=3.16,P＜0.05);其靶基因p63、生存素mRNA表达量分别为对照组的(4.79±0.63)和(3.43±0.46)倍,表达水平明显上调(t值分别为4.72、4.35,均P＜0.05);靶基因p63、生存素蛋白表达量分别为对照组的(2.40±0.23),(3.49±0.14)倍,表达水平也明显上调(t值分别为3.87、4.36,均P＜0.05).寻常性银屑病皮损中miR-203mRNA与生存素mRNA呈负相关(r=-0.36,P＜0.05),与p63 mRNA呈负相关(r=-0.43,P＜0.05).结论 miR-203及其下游靶基因p63、生存素可能参与了银屑病的发病过程.
目的 檢測尋常性銀屑病皮損中miR-203及其下遊靶基因p63、生存素的錶達水平.方法 實時PCR法檢測30例尋常性銀屑病患者皮損及30例健康對照皮膚中miR-203、p63、生存素mRNA的錶達水平,miR-203以U6為內參照,p63和生存素以GAPDH為內參照;Western印跡法檢測靶基因p63、生存素蛋白錶達水平,兩組間比較採用t檢驗,尋常性銀屑病皮損中miR-203與生存素、p63錶達的相關性採用Pearson相關分析.結果 與健康對照皮膚相比,尋常性銀屑病皮損中miR-203 mRNA錶達量為對照組的(0.41±0.11)倍,錶達明顯下調(t=3.16,P＜0.05);其靶基因p63、生存素mRNA錶達量分彆為對照組的(4.79±0.63)和(3.43±0.46)倍,錶達水平明顯上調(t值分彆為4.72、4.35,均P＜0.05);靶基因p63、生存素蛋白錶達量分彆為對照組的(2.40±0.23),(3.49±0.14)倍,錶達水平也明顯上調(t值分彆為3.87、4.36,均P＜0.05).尋常性銀屑病皮損中miR-203mRNA與生存素mRNA呈負相關(r=-0.36,P＜0.05),與p63 mRNA呈負相關(r=-0.43,P＜0.05).結論 miR-203及其下遊靶基因p63、生存素可能參與瞭銀屑病的髮病過程.
목적 검측심상성은설병피손중miR-203급기하유파기인p63、생존소적표체수평.방법 실시PCR법검측30례심상성은설병환자피손급30례건강대조피부중miR-203、p63、생존소mRNA적표체수평,miR-203이U6위내삼조,p63화생존소이GAPDH위내삼조;Western인적법검측파기인p63、생존소단백표체수평,량조간비교채용t검험,심상성은설병피손중miR-203여생존소、p63표체적상관성채용Pearson상관분석.결과 여건강대조피부상비,심상성은설병피손중miR-203 mRNA표체량위대조조적(0.41±0.11)배,표체명현하조(t=3.16,P＜0.05);기파기인p63、생존소mRNA표체량분별위대조조적(4.79±0.63)화(3.43±0.46)배,표체수평명현상조(t치분별위4.72、4.35,균P＜0.05);파기인p63、생존소단백표체량분별위대조조적(2.40±0.23),(3.49±0.14)배,표체수평야명현상조(t치분별위3.87、4.36,균P＜0.05).심상성은설병피손중miR-203mRNA여생존소mRNA정부상관(r=-0.36,P＜0.05),여p63 mRNA정부상관(r=-0.43,P＜0.05).결론 miR-203급기하유파기인p63、생존소가능삼여료은설병적발병과정.
Objective To measure the expressions of miR-203 and its downstream target genes p63 and survivin in psoriasis vulgaris lesions.Methods Tissue specimens were collected from lesions of 30 patients with psoriasis vulgaris and normal skin of 30 healthy human controls.Real-time PCR was performed to detect miR-203 mRNA expression with U6 as the internal control,as well as p63 and survivin mRNA expressions with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as the internal control,and Western blot to measure the protein expressions of miR-203 targets p63 and survivin.Statistical analysis was carried out by t test for intergroup comparisons and by Pearson correlation analysis for the analysis of correlation between miR-203,p63 and survivin expressions in psoriasis vulgaris lesions.Results Compared with the normal control skin,psoriasis vulgaris lesions showed significantly decreased mRNA expression level (2-△△C△△Ct) of miR-203 (0.41 ± 0.11,t =3.16,P ＜ 0.05),but increased mRNA expression levels of p63 (4.79 ± 0.63,t =4.72,P＜ 0.05) and survivin (3.43 ± 0.46,t =4.35,P＜ 0.05).The protein expression levels of p63 and survivin in these lesions were (2.40 ± 0.23) times (t =3.87,P ＜ 0.05) and (3.49 ± 0.14) times (t =4.36,P ＜ 0.05) those in the normal control skin respectively.In psoriasis vulgaris lesions,the mRNA expression level of miR-203 showed a significantly negative correlation with that of survivin (r =-0.36,P ＜ 0.05) and p63 (r =-0.43,P ＜ 0.05).Conclusion miR-203 and its downstream target genes p63 and survivin may participate in the occurrence of psoriasis vulgaris.
