生物安全学报
生物安全學報
생물안전학보
Journal of Biosafety
2015年
1期
57-63
,共7页
李丹%张震%王育鹏%秦娟%李为花%谭曼丽
李丹%張震%王育鵬%秦娟%李為花%譚曼麗
리단%장진%왕육붕%진연%리위화%담만려
入侵植物%喜旱莲子草%杀虫活性%植物源农药%资源化利用
入侵植物%喜旱蓮子草%殺蟲活性%植物源農藥%資源化利用
입침식물%희한련자초%살충활성%식물원농약%자원화이용
invasive species%Alternanthera philoxeroides%insecticidal activity%botanical pesticide%resource utilization
【背景】喜旱莲子草是全球性恶性入侵杂草,近年来在我国已呈蔓延之势,对经济及生态环境造成严重破坏。目前已开展了其入侵机制和生物防治等方面的研究,然而,针对其资源化利用的研究较少。【方法】分别研究了喜旱莲子草的石油醚、氯仿、乙酸乙酯和正丁醇等溶剂提取物对小菜蛾、斜纹夜蛾的选择忌避、触杀和胃毒作用。【结果】喜旱莲子草各有机相提取物对小菜蛾和斜纹夜蛾均具有一定的选择忌避作用。随着处理时间的延长,不同溶剂提取物对小菜蛾和斜纹夜蛾的触杀和胃毒作用效果逐渐增强,且校正死亡率与提取物浓度呈正相关关系;经喜旱莲子草石油醚提取物、乙酸乙酯提取物和正丁醇提取物4000 g· L-1处理后,小菜蛾的触杀校正死亡率均达到70%,经石油醚提取物、乙酸乙酯提取物、正丁醇提取物和水提取物4000 g· L-1及正丁醇层和水层500 g· L-1处理后,斜纹夜蛾的触杀和胃毒校正死亡率均达到80%。【结论与意义】该研究结果对喜旱莲子草的进一步研究开发具有参考价值。
【揹景】喜旱蓮子草是全毬性噁性入侵雜草,近年來在我國已呈蔓延之勢,對經濟及生態環境造成嚴重破壞。目前已開展瞭其入侵機製和生物防治等方麵的研究,然而,針對其資源化利用的研究較少。【方法】分彆研究瞭喜旱蓮子草的石油醚、氯倣、乙痠乙酯和正丁醇等溶劑提取物對小菜蛾、斜紋夜蛾的選擇忌避、觸殺和胃毒作用。【結果】喜旱蓮子草各有機相提取物對小菜蛾和斜紋夜蛾均具有一定的選擇忌避作用。隨著處理時間的延長,不同溶劑提取物對小菜蛾和斜紋夜蛾的觸殺和胃毒作用效果逐漸增彊,且校正死亡率與提取物濃度呈正相關關繫;經喜旱蓮子草石油醚提取物、乙痠乙酯提取物和正丁醇提取物4000 g· L-1處理後,小菜蛾的觸殺校正死亡率均達到70%,經石油醚提取物、乙痠乙酯提取物、正丁醇提取物和水提取物4000 g· L-1及正丁醇層和水層500 g· L-1處理後,斜紋夜蛾的觸殺和胃毒校正死亡率均達到80%。【結論與意義】該研究結果對喜旱蓮子草的進一步研究開髮具有參攷價值。
【배경】희한련자초시전구성악성입침잡초,근년래재아국이정만연지세,대경제급생태배경조성엄중파배。목전이개전료기입침궤제화생물방치등방면적연구,연이,침대기자원화이용적연구교소。【방법】분별연구료희한련자초적석유미、록방、을산을지화정정순등용제제취물대소채아、사문야아적선택기피、촉살화위독작용。【결과】희한련자초각유궤상제취물대소채아화사문야아균구유일정적선택기피작용。수착처리시간적연장,불동용제제취물대소채아화사문야아적촉살화위독작용효과축점증강,차교정사망솔여제취물농도정정상관관계;경희한련자초석유미제취물、을산을지제취물화정정순제취물4000 g· L-1처리후,소채아적촉살교정사망솔균체도70%,경석유미제취물、을산을지제취물、정정순제취물화수제취물4000 g· L-1급정정순층화수층500 g· L-1처리후,사문야아적촉살화위독교정사망솔균체도80%。【결론여의의】해연구결과대희한련자초적진일보연구개발구유삼고개치。
Background] Alternanthera philoxeroides is a globally distributed invasive weed, causing serious economic and ecologi-cal damage in China.[Method] To investigate the toxic activity of A.philoxeroides, we prepared extracts by petroleum ether, chloro-form, ethyl acetate and n-butyl alcohl.Repellency, contact and post-digestive toxicity of the extracts was tested on the lepidopteran pests Plutella xylostella and Spodoptera litura.[Result] ( 1) Each extract of A.philoxeroides had a repellent effect on both P.xylos-tella and S.litura.The effect of contact as well as post-digestive toxicity was stronger after longer extractin time;the corrected mor-tality rates and concentrations were positively correlated.(2) The corrected mortality of A.philoxeroides extracts by petroleum ether, ethyl acetate and n-butanol at a rate of 4000 g plant material/L of extraction agent on P.xylostella reached 70%, petroleum ether, ethyl acetate layer, n-butanol and water at a rate of of 4000 g plant material/L extracting agent and n-butanol and water layer of 500 g plant material/L extracting agent on S.litura reached 80%.[Conclusion and significance] The study had the reference value for the further research on A.philoxeroides and the development of new botanical pesticides.