CAJ | 학술논문

目的：探究电针足三里对老年痴呆小鼠大脑皮层β‐淀粉样肽前体蛋白（APP）及β‐淀粉样肽蛋白（Aβ）表达的影响。方法：将快速老化小鼠S A M P8随机分为模型组、电针组和非穴组，并设同源SAM R1小鼠作为对照组。电针组给予足三里电针干预，非穴组针刺双侧固定非穴位点。应用免疫组织化学SABC法观察各组小鼠大脑皮层APP及Aβ的表达情况。结果：SAMP8大脑皮层内APP和Aβ蛋白呈较高表达的状态，电针治疗后明显下调，与模型组、非穴组相比，差异有统计学意义（P＜0．01）；而非穴组与模型组相比，各指标差异无统计学意义（P＞0．05）。结论：电针足三里能有效改善AD ，其机制可能与其下调AD小鼠大脑皮层A PP及Aβ蛋白水平的表达有关。
목적：탐구전침족삼리대노년치태소서대뇌피층β‐정분양태전체단백（APP）급β‐정분양태단백（Aβ）표체적영향。방법：장쾌속노화소서S A M P8수궤분위모형조、전침조화비혈조，병설동원SAM R1소서작위대조조。전침조급여족삼리전침간예，비혈조침자쌍측고정비혈위점。응용면역조직화학SABC법관찰각조소서대뇌피층APP급Aβ적표체정황。결과：SAMP8대뇌피층내APP화Aβ단백정교고표체적상태，전침치료후명현하조，여모형조、비혈조상비，차이유통계학의의（P＜0．01）；이비혈조여모형조상비，각지표차이무통계학의의（P＞0．05）。결론：전침족삼리능유효개선AD ，기궤제가능여기하조AD소서대뇌피층A PP급Aβ단백수평적표체유관。
Objective:To investigate the expressing levels of APP and Aβ proteins owing to the influence of e‐lectro‐acupuncture at“Zusanli” in Seneseenee accelerated mice (SAMP8) brain .Methods :The Seneseenee accelerated mice (SAMP8) were randomLy divided into 3 groups :model group ,anelectro‐acupuncture group ,and sham‐acupuncture group .The SAMR1 was used as control .The expressing levels of APP and Aβproteins were evaluated by immunohisto‐chemical assays .Results :The expressing levels of APP and Aβproteins in the cortex of Seneseenee accelerated mice were high .They were both reduced by electro‐acupuncture compared with model group or sham‐acupuncture group . The differences were statistically significant(P<0 .01 ,P<0 .01) .Meanwhile ,there were no statistically significant difference between the various indicators in sham‐acupuncture group(P>0 .05 ,P>0 .05) .Conclusion:Alzheimer's disease could be treated effectively by Electro‐acupuncture at “Zusanli” .The mechanism was possibly related to the down‐regulated ex‐pressing levels of APP and Aβproteins in the cortex of SAMP8 .