CAJ | 학술논문

目的：：初步分析miR-181a与鼻咽癌细胞系C666-1放射敏感的相关性。方法：首先使用大剂量8 Gy的X射线照射C666-1,挑选三个大的存活亚克隆并命名为 C666-1-R1,C666-1-R2和 C666-1-R3,然后应用MTT等技术分析亚克隆及对照母本C666-1细胞系放射敏感性的差异,最后采用实时荧光定量PCR 法,分析三个存活亚克隆及对照C666-1的miR-181 a表达水平。结果：通过MTT比色法检测细胞增殖：6 Gy照射后,三个亚克隆C666-1-R1,C666-1-R2和C666-1-R3的细胞存活率均高于对照细胞C666-1,尤其C666-1-R2在48、72和96 h的存活率增高均有显著性差异(P<0.05),提示C666-1-R2具有放射抗拒性。另一方面,各亚克隆及对照细胞的 miR-181a 的2-△△Ct值如下：C666-1-R1=0.693,C666-1-R2=0.486,C666-1-R3=0.762,C666-1=1。提示放射后存活的三个亚克隆均高表达 miR-181a,尤其放射抗拒亚克隆C666-1-R2的 miR-181a表达更高。结论：miR-181a与鼻咽癌存活亚克隆的放射抗拒性密切相关。miR-181a可能是评估鼻咽癌放射敏感性的一个新分子,miR-181a 与鼻咽癌放射抗拒的相关性值得深入研究。
목적：：초보분석miR-181a여비인암세포계C666-1방사민감적상관성。방법：수선사용대제량8 Gy적X사선조사C666-1,도선삼개대적존활아극륭병명명위 C666-1-R1,C666-1-R2화 C666-1-R3,연후응용MTT등기술분석아극륭급대조모본C666-1세포계방사민감성적차이,최후채용실시형광정량PCR 법,분석삼개존활아극륭급대조C666-1적miR-181 a표체수평。결과：통과MTT비색법검측세포증식：6 Gy조사후,삼개아극륭C666-1-R1,C666-1-R2화C666-1-R3적세포존활솔균고우대조세포C666-1,우기C666-1-R2재48、72화96 h적존활솔증고균유현저성차이(P<0.05),제시C666-1-R2구유방사항거성。령일방면,각아극륭급대조세포적 miR-181a 적2-△△Ct치여하：C666-1-R1=0.693,C666-1-R2=0.486,C666-1-R3=0.762,C666-1=1。제시방사후존활적삼개아극륭균고표체 miR-181a,우기방사항거아극륭C666-1-R2적 miR-181a표체경고。결론：miR-181a여비인암존활아극륭적방사항거성밀절상관。miR-181a가능시평고비인암방사민감성적일개신분자,miR-181a 여비인암방사항거적상관성치득심입연구。
Objective:To investigate the correlation of miR-181a and the radiosensitive of C666-1 cell line from nasopharyngeal carcinoma.Methods:First,C666-1 was irradiated by using high dose 8 Gy X-ray.Three survival subclones were selected and named for C666-1-R1,C666-1-R2 and C666-1-R3;Then,the difference of cell radiosensitivity between survival subclonedand the control sample was analyzed by MTT assay;Finally,the expression of miR-181a between three survival subclones and control sample was analyzed by fluorescence quantitative PCR.Results:Cell proliferation was detected by MTT colorimetric assay.As compared with the control C666-1,the cell survival rate of C666-1-R1,C666-1-R2 and C666-1-R3 was higher by using 6 Gy X-ray,especially for C666-1-R2.The cell survival rate of C666-1-R2 differed significantly by X-ray in 48 h,72 h and 96 h(P<0.05).These results suggested that C666-1-R2 was radioresistance.On the other hand,the relative values of miR-181a (2-△△Ct )for each subclone and the control cells were showed as following:C666-1-R1=0.693,C666-1-R2=0.486,C666-1-R3=0.762,C666-1=1.These results suggested that miR-181 a of three survival subclones had high expression level, especially the radioresistance C666-1-R2.Conclusion:miR-181a and the radioresistance of survival subclones in nasopharyngeal carcinoma are closely related.miR-181a may be used as a new marker for assessing the radiosensitivity of nasopharyngeal carcinoma.The relation of miR-181a and the radioresistance of nasopharyngeal carcinoma are worthy of further study.