CAJ | 학술논문

目的：比较第2代杂交捕获技术（HC-2）和低温 PCR 检测法对宫颈病变的诊断应用价值。方法收集2013年4月－2014年4月来新疆医科大学附属肿瘤医院就诊的232例维吾尔族宫颈癌、宫颈上皮内瘤样病变（CIN）及慢性宫颈炎患者宫颈分泌物标本，分别进行 HC-2和低温 PCR 检测，低温 PCR 技术检测不同宫颈病变组织中 HPV 亚型，HC-2法测定病变组织中 HPV DNA 含量。分析2种方法检测结果的一致性。结果在232例标本中，低温 PCR 检测 HPV 阳性76例，阳性率为32．76％；HC-2检测 HPV 阳性70例，阳性率为30．17％。2种方法检测结果一致者210例，一致率为90．51％，Kappa 值为0．780；慢性宫颈炎患者 HPV 亚型以 HPV16、HPV56为主；CINⅠ患者 HPV 亚型以 HPV16、HPV18、HPV59为主；CINⅡ患者 HPV 亚型以 HPV16、HPV59为主；CINⅢ患者 HPV 亚型以 HPV16、HPV31为主；宫颈癌患者 HPV 亚型以 HPV16、HPV18、HPV58为主；CINⅠ～Ⅲ以及宫颈癌患者的 HPV DNA 含量均高于慢性宫颈炎患者（P ＜0．01），宫颈癌患者的 HPV DNA 含量高于 CINⅠ～Ⅲ，差异均有统计学意义（P ＜0．01）。结论 HC-2和低温 PCR 检测在宫颈严重病变中有较强的一致性，2种检测方法对宫颈病变的筛查均有较好的应用价值。
목적：비교제2대잡교포획기술（HC-2）화저온 PCR 검측법대궁경병변적진단응용개치。방법수집2013년4월－2014년4월래신강의과대학부속종류의원취진적232례유오이족궁경암、궁경상피내류양병변（CIN）급만성궁경염환자궁경분비물표본，분별진행 HC-2화저온 PCR 검측，저온 PCR 기술검측불동궁경병변조직중 HPV 아형，HC-2법측정병변조직중 HPV DNA 함량。분석2충방법검측결과적일치성。결과재232례표본중，저온 PCR 검측 HPV 양성76례，양성솔위32．76％；HC-2검측 HPV 양성70례，양성솔위30．17％。2충방법검측결과일치자210례，일치솔위90．51％，Kappa 치위0．780；만성궁경염환자 HPV 아형이 HPV16、HPV56위주；CINⅠ환자 HPV 아형이 HPV16、HPV18、HPV59위주；CINⅡ환자 HPV 아형이 HPV16、HPV59위주；CINⅢ환자 HPV 아형이 HPV16、HPV31위주；궁경암환자 HPV 아형이 HPV16、HPV18、HPV58위주；CINⅠ～Ⅲ이급궁경암환자적 HPV DNA 함량균고우만성궁경염환자（P ＜0．01），궁경암환자적 HPV DNA 함량고우 CINⅠ～Ⅲ，차이균유통계학의의（P ＜0．01）。결론 HC-2화저온 PCR 검측재궁경엄중병변중유교강적일치성，2충검측방법대궁경병변적사사균유교호적응용개치。
Objective To compare the diagnostic and applied value of hybrid capture 2 technology (HC-2) and low temperature PCR assay in cervical lesion.Methods Specimens of cervical secretions from 232 cases of cervical cancer,cervical intraepithelial neoplasia lesions (CIN)and chronic cervicitis were collected in our hospital.The specimens were detected by HC-2 and low temperature PCR assay and the consistency of the results of two methods was analyzed.Results The HPV positive rate of low temperature PCR de-tection was 32.76% (76/232)and HPV positive rate of HC-2 was 30.17% (70/232)in specimens of 232 cases.The consistent rate of two methods was 90.51% (210/232)and the Kappa value was 0.780;The main HPV subtypes of chronic cervicitis were HPV16,HPV56,and the main HPV subtypes of CINⅠwere HPV16,HPV18,HPV59,and the main HPV subtypes of CINⅡ were HPV16,HPV59,and the main HPV subtypes of CINⅢ were HPV16,HPV31,while the main HPV subtypes of cervical cancer were HPV16,HPV18,HPV58;The HPV DNA levels of CINⅠ-Ⅲ and cervical cancer were higher than chronic cervicitis (P <0.01),in addition,The HPV DNA levels of cervical cancer were higher than CINⅠ-Ⅲ (P <0.01).Conclusion The consistency of HC-2 and low temperature PCR detection in cervical se-vere lesions was strong and the two kinds of detection methods both had strong application value for the screening of cervical lesions.