CAJ | 학술논문

目的观察银屑病患者皮损和外周血中CD8α+α+T细胞的分布和比例,探讨其在银屑病发病中的作用.方法采用免疫荧光技术观察5例进展期寻常性银屑病患者和5例健康人皮肤中CD8α+α+T细胞的分布,流式细胞仪分别检测10例进展期寻常性银屑病患者和8例健康人外周血中CD8α+α+T细胞比例及干扰素γ(IFN-γ)和肿瘤坏死因子α(TNF-α)的表达.采用Graphpad Prism统计软件对实验组和对照组各数据进行t检验.结果在5例银屑病患者皮损真皮浅层均可见以CD8α+α+T细胞为主的浸润,未见明显的CD8α+β+T细胞浸润;而5例健康人皮肤中均未见CD8α+α+T细胞和CD8α+β+T细胞浸润.流式细胞仪检测,8例健康人外周血中CD8α+α+T细胞比例为9.12％±4.80％,10例银屑病患者组为26.47％±12.99％,银屑病患者组明显高于对照组,两组比较,t=3.96,P＜ 0.001.在银屑病患者外周血CD8α+α+T细胞中分泌IFN-γ和TNF-α的细胞比例分别为47.36％±19.38％和54.14％±21.14％,健康对照组分别为13.44％±9.21％和34.03％±17.22％,两组差异均有统计学意义(t值分别为4.54和2.17,P值分别＜0.001和＜0.05).结论银屑病患者皮损和外周血中存在CD8α+α+T细胞的分布和比例增多,CD8α+α+T细胞可能是参与银屑病发病的主要CD8+T细胞亚群.
목적 관찰은설병환자피손화외주혈중CD8α+α+T세포적분포화비례,탐토기재은설병발병중적작용.방법 채용면역형광기술관찰5례진전기심상성은설병환자화5례건강인피부중CD8α+α+T세포적분포,류식세포의분별검측10례진전기심상성은설병환자화8례건강인외주혈중CD8α+α+T세포비례급간우소γ(IFN-γ)화종류배사인자α(TNF-α)적표체.채용Graphpad Prism통계연건대실험조화대조조각수거진행t검험.결과 재5례은설병환자피손진피천층균가견이CD8α+α+T세포위주적침윤,미견명현적CD8α+β+T세포침윤;이5례건강인피부중균미견CD8α+α+T세포화CD8α+β+T세포침윤.류식세포의검측,8례건강인외주혈중CD8α+α+T세포비례위9.12％±4.80％,10례은설병환자조위26.47％±12.99％,은설병환자조명현고우대조조,량조비교,t=3.96,P＜ 0.001.재은설병환자외주혈CD8α+α+T세포중분비IFN-γ화TNF-α적세포비례분별위47.36％±19.38％화54.14％±21.14％,건강대조조분별위13.44％±9.21％화34.03％±17.22％,량조차이균유통계학의의(t치분별위4.54화2.17,P치분별＜0.001화＜0.05).결론 은설병환자피손화외주혈중존재CD8α+α+T세포적분포화비례증다,CD8α+α+T세포가능시삼여은설병발병적주요CD8+T세포아군.
Objective To investigate the distribution and proportion of CD8α+α + T cells in lesions and peripheral blood of patients with psoriasis,and to assess their roles in the pathogenesis of psoriasis.Methods An immunofluorescence assay was performed to observe the distribution of CD8α+α+ T cells in lesions of 5 patients with progressive psoriasis vulgaris and normal skin of 5 healthy human controls.Flow cytometry was conducted to determine the proportion of CD8α+α+ T cells,and to measure the expressions of interferon (IFN)-γ and tumor necrosis factor (TNF)-α in peripheral blood from 10 patients with progressive psoriasis vulgaris and 8 healthy human controls.Statistical analysis was carried out by t test with GraphPad Prism software.Results A massive infiltrate mainly composed of CD8α+α+ T cells but not CD8α+β+ T cells was observed in the upper dermis of lesions from the 5 patients with psoriasis,while there was no infiltrate of CD8α+β+ or CD8α+α+ T cells in the normal skin of 5 healthy human controls.Flow cytometry revealed that the proportion of CD8α+α+ T cells was significantly higher in peripheral blood from 10 patients with psoriasis than in that from 8 healthy human controls (26.47％ ± 12.99％ vs.9.12％ ± 4.80％,t =3.96,P＜ 0.001).Significant differences were also noted between the psoriatic patients and healthy human controls in the percentage of cells secreting IFN-γ (47.36％ ± 19.38％ vs.13.44％ ± 9.21％,t =4.54,P ＜ 0.001) and cells secreting TNF-α (54.14％ ± 21.14％ vs.34.03％ ± 17.22％,t =2.17,P ＜ 0.05) in peripheral blood CD8α+α+ T cells.Conclusions Both the distribution and proportion of CD8α+α+ T cells are increased in lesions and peripheral blood from patients with psoriasis,suggesting that CD8α+α+ T cells may be the main subgroup of CD8+ T cells that contribute to the pathogenesis of psoriasis.