CAJ | 학술논문

目的：建立同时、快速、准确检测番茄酱中19种常见农药残留量的超高效液相色谱-串联四级杆质谱(UPLC-MS/MS)分析方法。方法番茄酱原料样品经水分散后用含1%乙酸的乙腈超声辅助提取,应用GCB和PSA 等吸附填料进行分散式固相萃取净化,离心后浓缩定容,最后使用超高效液相色谱-串联四级杆质谱仪进行定性和外标法定量。结果19种农药在0.001~0.10 mg/L浓度范围内呈现良好线性关系,r>0.994；在0.010、0.050、0.10 mg/kg三个添加浓度上回收率为72.2%~116.8%,相对标准偏差小于13%,检出限在0.016~3.96μg/kg。结论该方法操作简单,快速可靠,重复性好,可满足国内外对番茄酱中19种农药检测的要求。
목적：건립동시、쾌속、준학검측번가장중19충상견농약잔류량적초고효액상색보-천련사급간질보(UPLC-MS/MS)분석방법。방법번가장원료양품경수분산후용함1%을산적을정초성보조제취,응용GCB화PSA 등흡부전료진행분산식고상췌취정화,리심후농축정용,최후사용초고효액상색보-천련사급간질보의진행정성화외표법정량。결과19충농약재0.001~0.10 mg/L농도범위내정현량호선성관계,r>0.994；재0.010、0.050、0.10 mg/kg삼개첨가농도상회수솔위72.2%~116.8%,상대표준편차소우13%,검출한재0.016~3.96μg/kg。결론해방법조작간단,쾌속가고,중복성호,가만족국내외대번가장중19충농약검측적요구。
Objective To develop a fast and accurate method for simultaneous determination of 19 pesticide residues in tomato paste by ultra performance liquid chromatography-tandem mass spectrometry. Methods Tomato paste was firstly dispersed by water and extracted with acetonitrile containing 1% acetic acid by ultra-sonic, and the extracts were purified by dispersive solid phase extraction using GCB and PSA as the sorbents. After centrifugation and concentrated by nitrogen flushing, samples were detected by UPLC-MS/MS.ResultsThe method showed a good linearity in the range of 0.001~0.10 mg/L for 19 pesticides withr>0.994. The recoveries were in the range of 72.2%~116.8% at 3 spiked levels of 0.010, 0.050, and 0.10 mg/kg for 19 pesticides, and the relative standard deviations (RSDs) were less than 13%. The limits of detections were in the range of 0.016~3.96μg/kg.Conclusion The method is simple, fast and reliable, with a good repeatability, which could meet the detection requirements of these 19 pesticide residues in tomato paste at home and abroad. The method is suitable for quality control and food safety risk control in factory.