黑龙江医药
黑龍江醫藥
흑룡강의약
HEILONGJIANG MEDICAL JOURNAL
2015年
2期
219-220,221
,共3页
HPLC%清热解毒片%黄芩苷
HPLC%清熱解毒片%黃芩苷
HPLC%청열해독편%황금감
s:HPLC%Qingre Jiedu tablets%Baicalin
目的：∶建立HPLC法测定清热解毒片中黄芩苷的含量测定方法。方法∶采用Diamonsil C18色谱柱(250 mm×4.6 mm，5μm)，以甲醇︰水︰冰醋酸（45︰55︰1）为流动相，检测波长为274nm，流速为1mL·min-1，进样量为10μL。结果∶黄芩苷浓度在0.02～0.1mg·mL-1范围内与峰面积呈良好的线性关系(r＝0.9999)，平均回收率为100.75%(n＝6)，RSD＝2.88%。结论∶HPLC法专属性强，灵敏度高，含量测定方法准确可靠，适用于该产品的质量检测。
目的：∶建立HPLC法測定清熱解毒片中黃芩苷的含量測定方法。方法∶採用Diamonsil C18色譜柱(250 mm×4.6 mm，5μm)，以甲醇︰水︰冰醋痠（45︰55︰1）為流動相，檢測波長為274nm，流速為1mL·min-1，進樣量為10μL。結果∶黃芩苷濃度在0.02～0.1mg·mL-1範圍內與峰麵積呈良好的線性關繫(r＝0.9999)，平均迴收率為100.75%(n＝6)，RSD＝2.88%。結論∶HPLC法專屬性彊，靈敏度高，含量測定方法準確可靠，適用于該產品的質量檢測。
목적：∶건립HPLC법측정청열해독편중황금감적함량측정방법。방법∶채용Diamonsil C18색보주(250 mm×4.6 mm，5μm)，이갑순︰수︰빙작산（45︰55︰1）위류동상，검측파장위274nm，류속위1mL·min-1，진양량위10μL。결과∶황금감농도재0.02～0.1mg·mL-1범위내여봉면적정량호적선성관계(r＝0.9999)，평균회수솔위100.75%(n＝6)，RSD＝2.88%。결론∶HPLC법전속성강，령민도고，함량측정방법준학가고，괄용우해산품적질량검측。
Objective:HPLC method was used to detect the content of Baicalin in Qingre Jiedu tablets. Method:Diamonsil C18 chromatographic column (250 mm × 4.6 mm,5μm)was used,the mobile phase was consisted of Methanol-water-Glacial acetic acid (45︰55︰1)with UV detection wavelength at 274nm,flow rate is 1mL·min-1,Sample size is 10μL. Result:The calibration curve was in good linearity between the score of peak area and concentration over the range of 0.02-0.1mg·mL(r=0.9999),the average recov-erey was 100.75%(n=6),RSD was 2.88%. Conclusion:This method is accurate、reliable、specific and reproducible,reliable to detect the content and is suitable to control the quality of Qingkailing tablets.
