CAJ | 학술논문

以体外培养的hGCCs MGC－803细胞系为研究对象，采用不同浓度石蒜碱（5、2．5、1．25μmol／L）进行处理，倒置显微镜下观察其生长状态，同时采用血球计数板统计其体外增殖率，采用流式细胞技术检测处于不同细胞周期的细胞比例．结果表明，石蒜碱处理对hGCCs的体外增殖具有一定的抑制作用，其中5μmol／L石蒜碱对hGCCs的抑制作用最为明显．显微观察和计数分析结果表明，5μmol／L石蒜碱处理的hGCCs活力最低，细胞凋亡现象最明显．细胞周期检测的结果显示石蒜碱处理会使hGCCs增殖趋缓，细胞周期延长．
이체외배양적hGCCs MGC－803세포계위연구대상，채용불동농도석산감（5、2．5、1．25μmol／L）진행처리，도치현미경하관찰기생장상태，동시채용혈구계수판통계기체외증식솔，채용류식세포기술검측처우불동세포주기적세포비례．결과표명，석산감처리대hGCCs적체외증식구유일정적억제작용，기중5μmol／L석산감대hGCCs적억제작용최위명현．현미관찰화계수분석결과표명，5μmol／L석산감처리적hGCCs활력최저，세포조망현상최명현．세포주기검측적결과현시석산감처리회사hGCCs증식추완，세포주기연장．
The hGCCs were treated with different concentration of lycorisradiata alkali (5、2.5、1.25 μmol/L re-spectively), growth status were observed by the inverted microscope, and proliferation rate were count by hemocytome-ter, the proportion of hGCCs in different cell cycles were detected by flow cytometry.The results showed that the prolif-eration of hGCCs was suppressed by treated with lycorisradiata alkali, the most obvious suppressed of hGCCs was 5μmol/L treatment group.Through microscopic observation and counting analysis results, it has been found that the vi-tality of hGCCs was the lowest and the cells apoptosis was the most obvious by treated of 5μmol/L lycorisradiata alka-li.The result of cell cycle detection revealed that by treated with lycorisradiata alkali, the reproduction of hGCCs be-came slow and cell cycle became extended.