CAJ | 학술논문

建立了超高效液相色谱法测定氟喹诺酮类药物粉剂中非法添加喹乙醇、乙酰甲喹、卡巴氧及喹烯酮的方法。采用purospher RP－18色谱柱（2．1 mm×100 mm，粒径2．0μm ）分离四种喹噁啉类药物，以磷酸溶液和甲醇－乙腈（7．5∶7．0）为流动相进行梯度洗脱，流速0．3 mL／min，二极管阵列检测器检测，采集波长范围为200～400 nm，分辨率为1．2 nm，记录光谱图和365 nm波长处的色谱图。结果显示，四种喹噁啉类药物的浓度在0．2～100μg／mL范围内的线性良好，相关系数r均为0．9999，回收率在98．0％～100．2％范围内，RSD在0．27％～0．89％之间，检测限200 mg／kg。本方法快速、准确，可用于氟喹诺酮类粉剂中非法添加喹噁啉类药物的定性和定量检测。
건립료초고효액상색보법측정불규낙동류약물분제중비법첨가규을순、을선갑규、잡파양급규희동적방법。채용purospher RP－18색보주（2．1 mm×100 mm，립경2．0μm ）분리사충규오람류약물，이린산용액화갑순－을정（7．5∶7．0）위류동상진행제도세탈，류속0．3 mL／min，이겁관진렬검측기검측，채집파장범위위200～400 nm，분변솔위1．2 nm，기록광보도화365 nm파장처적색보도。결과현시，사충규오람류약물적농도재0．2～100μg／mL범위내적선성량호，상관계수r균위0．9999，회수솔재98．0％～100．2％범위내，RSD재0．27％～0．89％지간，검측한200 mg／kg。본방법쾌속、준학，가용우불규낙동류분제중비법첨가규오람류약물적정성화정량검측。
A method for the determination of olaquindox, maquindox, carbadox, quinocetone in quinolones powder by UPLC was developed. Purospher RP-18 column ( 2. 1 mm × 100 mm, 2. 0 μm) was used with the mobile phase consisted of phosphoric acid solution and methanol-acetonitrile ( 7. 5 ∶ 7. 0 ) . The flow rate was 0.3 mL/min; the column temperature was 30℃;the wavelength range was 200~400 nm. The result showed that the standard curves for four quinoxalines were in good linearity within a concentration range of 0.2~100μg/mL( r=0.9999) , the recoveries for quinoxalines in quinolones powder ranged from 98.0%to 100.2%with the RSD from 0.27%to 0.89%. The limit of detection( LOD) was 200 mg/kg. The method was fast and accurate, and was suited for identification and determination of the quinoxalines in quinolones powder.