CAJ | 학술논문

目的：建立一种饲料中26种β2-受体激动剂兽药残留的液相色谱-串联质谱(HPLC-MS/MS)检测方法。方法样品经盐酸甲醇提取液提取，醋酸铅沉淀蛋白后，过混合阳离子(MCX)固相萃取柱净化。采用0.1%甲酸(A)和0.1%甲酸-乙腈(B)作为流动相进行梯度洗脱，质谱(ESI+)采用多离子检测模式(MRM)对β2-受体激动剂的定量离子和定性离子进行监测。结果本方法在15 min内完成26种目标化合物的分离分析。26种β2-受体激动剂在20、50和100μg/L添加水平的回收率为71.9%~102.9%，相对标准偏差小于11.8%(n=6)，方法定量限为10μg/kg。结论该方法快速、准确、灵敏，适合测定饲料中的β2-受体激动剂类药物残留。
목적：건립일충사료중26충β2-수체격동제수약잔류적액상색보-천련질보(HPLC-MS/MS)검측방법。방법양품경염산갑순제취액제취，작산연침정단백후，과혼합양리자(MCX)고상췌취주정화。채용0.1%갑산(A)화0.1%갑산-을정(B)작위류동상진행제도세탈，질보(ESI+)채용다리자검측모식(MRM)대β2-수체격동제적정량리자화정성리자진행감측。결과본방법재15 min내완성26충목표화합물적분리분석。26충β2-수체격동제재20、50화100μg/L첨가수평적회수솔위71.9%~102.9%，상대표준편차소우11.8%(n=6)，방법정량한위10μg/kg。결론해방법쾌속、준학、령민，괄합측정사료중적β2-수체격동제류약물잔류。
Objective To determination of26β2-agonist residues in feed samples by liquid chromatography -tandem mass spectrometric (LC–MS/MS).MethodsFeed samples were extracted with muriatic acid and methanol solution. And then theβ2-agonist residues in the extracts were separated on a reversed phase using a gradient elution program of 0.1% formic acid aqueous solution (A) and 0.1% formic acid in acetonitrile solution (B). Using LC–MS/MS (ESI+) with multiple reactions monitoring (MRM), identification of the major components of the 26 kinds ofβ2-agonist residues was performed based upon the intensities of fragments. ResultsThe recoveries were ranged from 71.9% to 102.9% for the 26 kinds ofβ2-agonist residues with 3 spiked levels of 20, 50 and 100μg/kg. The relative standard deviations (RSDs) were less than 11.8% (n=6), and the limits of quantitation (LOQ) for the 26 kinds ofβ2-agonists were 10μg/kg.ConclusionThe proposed method is fast, accurate and sensitive, which is suitable for detectingβ2-agonist residues in feed samples.