CAJ | 학술논문

目的：建立一种采用高效液相色谱同时检测食品中苯甲酸、山梨酸、脱氢乙酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯等7种防腐剂的方法,并对葡萄酒、饮料、酱油、面包等4种基质进行测定。方法样品经甲醇提取,无水硫酸镁除水离心后取上清液经0.45μm滤膜过滤,使用高效液相色谱二极管阵列检测器(DAD)对7种防腐剂含量进行测定。结果在优选条件下,方法线性范围为0.1~10 mg/L,线性相关系数R2≥0.9999,检出限(S/N=3)为50~100μg/L,样品回收率为77.28%~106.81%,相对标准偏差(RSD)为0.46%~2.38%。结论该方法操作简便,灵敏度高,适用于葡萄酒、饮料、酱油和面包4种基质中7种防腐剂的检测。
목적：건립일충채용고효액상색보동시검측식품중분갑산、산리산、탈경을산、대간기분갑산갑지、대간기분갑산을지、대간기분갑산병지、대간기분갑산정지등7충방부제적방법,병대포도주、음료、장유、면포등4충기질진행측정。방법양품경갑순제취,무수류산미제수리심후취상청액경0.45μm려막과려,사용고효액상색보이겁관진렬검측기(DAD)대7충방부제함량진행측정。결과재우선조건하,방법선성범위위0.1~10 mg/L,선성상관계수R2≥0.9999,검출한(S/N=3)위50~100μg/L,양품회수솔위77.28%~106.81%,상대표준편차(RSD)위0.46%~2.38%。결론해방법조작간편,령민도고,괄용우포도주、음료、장유화면포4충기질중7충방부제적검측。
Objective To establish a method for the simultaneous determination of 7 preservatives (benzoic acid, sorbic acid, dehydroacetic acid, methyl p-hydroxybenzoate, p-hydroxy benzoic acid ethyl ester, p-hydroxy benzoic acid n-propyl, and p-hydroxy benzoic acid butyl ester) in food by high performance liquid chromatography, and determine the wine, drink, soy sauce, and bread in the samples.MethodsSamples were extracted with methanol, and assisted by MgSO4 to remove the water before the centrifugation. After through 0.45μm filter, 7 kinds of preservatives were detected by HPLC-DAD.ResultsUnder the optimized conditions, the linear range was 0.1~ 10 mg/L withR2≥0.9999. The limits of detection (LOD,S/N=3) were from 50 to 100μg/L. Besides, recoveries were in the range of 77.28%~106.81%, and the RSDs of the peak areas were between 0.46%~2.38%.ConclusionThis method is suitable for simple and sensitive determination of 7 kinds of preservatives in wine, drink, soy sauce and bread.