CAJ | 학술논문

建立了高效液相色谱法测定预混合饲料、配合饲料和浓缩饲料中艾司唑仑含量的检测方法。试样用正己烷－二氯甲烷（1∶1，V／V）提取，经MCX固相萃取柱进一步净化，采用反相高效液相色谱－紫外检测法进行定量分析。饲料中艾司唑仑的检测限为0．1 mg／kg，定量限为0．25 mg／kg。在饲料中添加0．25、0．5、2．5 mg／kg水平的药物回收试验中，平均回收率均在82．8％～105．9％之间，相对标准偏差为1．62％～8．40％。该方法灵敏、准确，适用于饲料中艾司唑仑含量的分析检测。
건립료고효액상색보법측정예혼합사료、배합사료화농축사료중애사서륜함량적검측방법。시양용정기완－이록갑완（1∶1，V／V）제취，경MCX고상췌취주진일보정화，채용반상고효액상색보－자외검측법진행정량분석。사료중애사서륜적검측한위0．1 mg／kg，정량한위0．25 mg／kg。재사료중첨가0．25、0．5、2．5 mg／kg수평적약물회수시험중，평균회수솔균재82．8％～105．9％지간，상대표준편차위1．62％～8．40％。해방법령민、준학，괄용우사료중애사서륜함량적분석검측。
A confirmatory method has been developed and validated for the determination of estazolam ( EZL) in premix, compound feed and concentrate feed using high performance liquid chromatography( HPLC) . The analyte was extracted from feeds using hexane-dichloromethane (1 ∶ 1, V/V), and then the sample extracts were purified by MCX solid-phase extraction. The analyte was analyzed by HPLC-UV. The limit of detection was 0.1 mg/kg for EZL, with quantification limit of 0.25 mg/kg. Recoveries were in the range of from 82.8% to 105.9% from feeds fortified at the 0.25, 0.5, 2.5 mg/kg levels, and the mean intra-laboratory reproducibility ranged from 1.62% to 8.40%. The developed method was sensitive and specific for the detection of estazolam in feeds.