检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2015年
4期
363-366
,共4页
胶体金免疫层析法%NS1抗原%IgG/IgM抗体%聚合酶链反应-荧光探针法%登革热病毒
膠體金免疫層析法%NS1抗原%IgG/IgM抗體%聚閤酶鏈反應-熒光探針法%登革熱病毒
효체금면역층석법%NS1항원%IgG/IgM항체%취합매련반응-형광탐침법%등혁열병독
Gold immunochromatographic assay%NS1antigen%IgG/IgM antibody%Polymerase chain reaction-fluorescent probe method%Dengue virus
目的：采用胶体金免疫层析法（ GICA）检测登革热病毒（ DENV） NS1抗原和／或IgG／IgM抗体，初步评价其临床应用效果。方法将2633例经聚合酶链反应（ PCR）-荧光探针法检测DENV核酸的血清样本分为3组：Ⅰ组为948例样本，用GICA检测NS1抗原；Ⅱ组为1156例样本，用GICA检测IgG／IgM抗体；Ⅲ组为529例样本，用GICA同时检测NS1抗原和IgG／IgM抗体。另5例流行性出血热抗体阳性样本、4例风疹／麻疹抗体阳性样本和50名健康人群样本为Ⅳ组，用GICA检测NS1抗原和IgG／IgM抗体。结果Ⅰ组结果与PCR结果相比，阳性、阴性、总体符合率分别为89．09％、92．41％、89．87％（P＜0．01）；Ⅱ组结果与PCR结果相比，阳性、阴性、总体符合率分别为64．68％、66．41％、64．88％（P＜0．01）；Ⅲ组结果与PCR结果相比，阳性、阴性、总体符合率分别为93．56％、61．82％、86．96％（P＞0．05），Ⅲ组中有419例样本经PCR检测结果为阳性， NS1抗原阳性率为85．20％，IgG／IgM抗体阳性率为63．25％，NS1抗原和／或IgG／IgM抗体阳性符合率为93．57％；Ⅳ组结果均为阴性，特异性为100％。结论 GICA对NS1抗原和IgG／IgM抗体的联合检测结果与PCR检测结果较接近，且特异性良好，可用于DENV感染的早期辅助诊断和筛查。
目的：採用膠體金免疫層析法（ GICA）檢測登革熱病毒（ DENV） NS1抗原和／或IgG／IgM抗體，初步評價其臨床應用效果。方法將2633例經聚閤酶鏈反應（ PCR）-熒光探針法檢測DENV覈痠的血清樣本分為3組：Ⅰ組為948例樣本，用GICA檢測NS1抗原；Ⅱ組為1156例樣本，用GICA檢測IgG／IgM抗體；Ⅲ組為529例樣本，用GICA同時檢測NS1抗原和IgG／IgM抗體。另5例流行性齣血熱抗體暘性樣本、4例風疹／痳疹抗體暘性樣本和50名健康人群樣本為Ⅳ組，用GICA檢測NS1抗原和IgG／IgM抗體。結果Ⅰ組結果與PCR結果相比，暘性、陰性、總體符閤率分彆為89．09％、92．41％、89．87％（P＜0．01）；Ⅱ組結果與PCR結果相比，暘性、陰性、總體符閤率分彆為64．68％、66．41％、64．88％（P＜0．01）；Ⅲ組結果與PCR結果相比，暘性、陰性、總體符閤率分彆為93．56％、61．82％、86．96％（P＞0．05），Ⅲ組中有419例樣本經PCR檢測結果為暘性， NS1抗原暘性率為85．20％，IgG／IgM抗體暘性率為63．25％，NS1抗原和／或IgG／IgM抗體暘性符閤率為93．57％；Ⅳ組結果均為陰性，特異性為100％。結論 GICA對NS1抗原和IgG／IgM抗體的聯閤檢測結果與PCR檢測結果較接近，且特異性良好，可用于DENV感染的早期輔助診斷和篩查。
목적：채용효체금면역층석법（ GICA）검측등혁열병독（ DENV） NS1항원화／혹IgG／IgM항체，초보평개기림상응용효과。방법장2633례경취합매련반응（ PCR）-형광탐침법검측DENV핵산적혈청양본분위3조：Ⅰ조위948례양본，용GICA검측NS1항원；Ⅱ조위1156례양본，용GICA검측IgG／IgM항체；Ⅲ조위529례양본，용GICA동시검측NS1항원화IgG／IgM항체。령5례류행성출혈열항체양성양본、4례풍진／마진항체양성양본화50명건강인군양본위Ⅳ조，용GICA검측NS1항원화IgG／IgM항체。결과Ⅰ조결과여PCR결과상비，양성、음성、총체부합솔분별위89．09％、92．41％、89．87％（P＜0．01）；Ⅱ조결과여PCR결과상비，양성、음성、총체부합솔분별위64．68％、66．41％、64．88％（P＜0．01）；Ⅲ조결과여PCR결과상비，양성、음성、총체부합솔분별위93．56％、61．82％、86．96％（P＞0．05），Ⅲ조중유419례양본경PCR검측결과위양성， NS1항원양성솔위85．20％，IgG／IgM항체양성솔위63．25％，NS1항원화／혹IgG／IgM항체양성부합솔위93．57％；Ⅳ조결과균위음성，특이성위100％。결론 GICA대NS1항원화IgG／IgM항체적연합검측결과여PCR검측결과교접근，차특이성량호，가용우DENV감염적조기보조진단화사사。
Objective To examine the Dengue virus (DENV) NS1 antigen and (or)the IgG/IgM antibody by gold immunochromatographic assay ( GICA) , and evaluate its preliminary clinical application effect .Methods 2 633 serum samples of which DENV nucleic acid has been tested by polymerase chain reaction ( PCR)-fluorescence probe technique were separated into 3 groups:948 samples of NS1 antigen in group Ⅰ, 1 156 samples of IgG/IgM antibody in group Ⅱ, and 529 samples of both NS1 antigen and IgG/IgM antibody in group Ⅲ have been tested by GICA.In addition, NS1 antigen and IgG/IgM antibody of 5 epidemic hemorrhagic fever antibody positive samples , 4 rubella/measles antibody positive samples as well as 50 samples of healthy subjects as group Ⅳ have also been tested by the same technique . Results The positive, negative and total coincidence rate of group Ⅰcompared to that of PCR were 89.09%, 92.41%and 89.87%respectively ( P<0.01); The positive, negative and total coincidence rate of group Ⅱ were 64.68%, 66.41%and 64.88%respectively (P<0.01);The positive, negative and total coincidence rate of group Ⅲwere 64. 68%, 66.41%and 64.88%(P>0.05).Among group Ⅲ, 419 samples showed positive by PCR .The positive rate of NS1 antigen, IgG/IgM antibody and both (or either)were 85.20%, 63.25%and 93.57%respectively.All results of groupⅣ showed negative , and the specificity was 100%.Conclusions The union test results of the DENV NS 1 antigen and the IgG/IgM antibody are comparable with those of PCR and the specificity is good .Therefore, it may be used for early auxiliary diagnosis and screening of the DENV .
