CAJ | 학술논문

为探讨空间环境对大肠杆菌生物学性状的影响，通过神舟十号飞船将大肠杆菌送至外太空（太空组），地面设立同步温度对照组，返回后进行菌落计数，观察太空飞行对细菌繁殖的影响；进行亚胺培南敏感性检测，了解耐药性变化；进行生长曲线测定，了解飞行后的生长速率。结果发现太空组细菌量为7.9×1010 cfu／mL，对照组为28.5×1010 cfu／mL；太空组平均抑菌圈直径为15.57±1.2 mm；对照组为13.4±1.15 mm；两组菌的生长曲线没有明显差异。研究表明大肠杆菌在太空飞行环境中生长受抑制，且对亚胺培南耐药性降低。
위탐토공간배경대대장간균생물학성상적영향，통과신주십호비선장대장간균송지외태공（태공조），지면설립동보온도대조조，반회후진행균락계수，관찰태공비행대세균번식적영향；진행아알배남민감성검측，료해내약성변화；진행생장곡선측정，료해비행후적생장속솔。결과발현태공조세균량위7.9×1010 cfu／mL，대조조위28.5×1010 cfu／mL；태공조평균억균권직경위15.57±1.2 mm；대조조위13.4±1.15 mm；량조균적생장곡선몰유명현차이。연구표명대장간균재태공비행배경중생장수억제，차대아알배남내약성강저。
To manifest the effect of space flight on biological characteristic in Escherichia coli , the strain was carried to space by Shenzhou-10 spaceship .The colony forming units ( CFU) in both the space flight and ground cultured E .coli were assessed by plate clone count respectively to calculate the survival rate of space flight E .coli sample .Antibiotics sensitivity for space flight and ground cul-tured E.coli was analyzed to compare the sensitivity to imipenem .Then the growth curves of the two strains were measured.The results show that, after space flight, the CFU of E.coli was 7.9 ×1010 cfu/mL, while it was 28.5 ×1010 cfu/mL in ground cultured E .coli.The average inhibition zone of imipenem for ground cultured E.coli was 13.4 ±1.15 mm in diameter, while that of the space flight gruop was 15.57 ±1.2 mm in diameter.However, the growth curves of the two strains had no sig-nificant difference .All data revealed that the growth of E .coli was inhibited in space flight environ-ment, and it could become less resistant to imipenem after space flight .