信阳师范学院学报（自然科学版）
信暘師範學院學報（自然科學版）
신양사범학원학보（자연과학판）
JOURNAL OF XINYANG NORMAL UNIVERSITY(NATURAL SCIENCE EDITION)
2015年
2期
244-247
,共4页
薤白多糖%羧甲基化修饰%响应面法优化%抗氧化活性
薤白多糖%羧甲基化脩飾%響應麵法優化%抗氧化活性
해백다당%최갑기화수식%향응면법우화%항양화활성
Allium macrosttemon Bge.polysaccharides( PAM)%carboxymethylated modification%response surface methodology( RSM)%antioxidative activity
采用氯乙酸法对醇沉法得到的薤白多糖（ PAM）和柱层析纯化的3种分级薤白多糖进行羧甲基化修饰，以氯乙酸浓度、反应时间和反应温度为自变量，修饰产物的羧甲基取代度（ DS）为响应值，应用响应面设计法确定羧甲基化修饰的最佳条件，用H2 O2／Fe2＋体系法和邻苯三酚自氧化法测定修饰产物的抗氧化活性．结果表明：薤白多糖氯乙酸法修饰的最佳条件为氯乙酸浓度1．3 mol／L、反应温度63℃、反应时间3．2 h．此条件下羧甲基取代度为0．882．羧甲基化修饰能提高薤白多糖的体外抗氧化活性．
採用氯乙痠法對醇沉法得到的薤白多糖（ PAM）和柱層析純化的3種分級薤白多糖進行羧甲基化脩飾，以氯乙痠濃度、反應時間和反應溫度為自變量，脩飾產物的羧甲基取代度（ DS）為響應值，應用響應麵設計法確定羧甲基化脩飾的最佳條件，用H2 O2／Fe2＋體繫法和鄰苯三酚自氧化法測定脩飾產物的抗氧化活性．結果錶明：薤白多糖氯乙痠法脩飾的最佳條件為氯乙痠濃度1．3 mol／L、反應溫度63℃、反應時間3．2 h．此條件下羧甲基取代度為0．882．羧甲基化脩飾能提高薤白多糖的體外抗氧化活性．
채용록을산법대순침법득도적해백다당（ PAM）화주층석순화적3충분급해백다당진행최갑기화수식，이록을산농도、반응시간화반응온도위자변량，수식산물적최갑기취대도（ DS）위향응치，응용향응면설계법학정최갑기화수식적최가조건，용H2 O2／Fe2＋체계법화린분삼분자양화법측정수식산물적항양화활성．결과표명：해백다당록을산법수식적최가조건위록을산농도1．3 mol／L、반응온도63℃、반응시간3．2 h．차조건하최갑기취대도위0．882．최갑기화수식능제고해백다당적체외항양화활성．
Total PAM extracted by ethanol precipitation and three hierarchical PAMs extracted by chromatography purified were carboxymethylation modified by chloroacetic acid method.With chloroacetic acid concentration, reaction time and reaction temperature as the independent variables and carboxymethyl substitution degree ( DS ) of modified product as the response value,the modification conditions were optimized by response surface analysis.The antioxidative activity of modified product was tested by H2 O2/Fe2+system and pyrogallol oxidation.The results showed that the opti-mal modification conditions were 1.3 mol/L of chloroacetic acid, the reaction temperature was 63℃and reaction time was 3.2 hours.Under these conditions,the obtained DS was 0.882.Carboxymethylated modification can enhance the antioxidative activity of PAM.
