检验医学
檢驗醫學
검험의학
LABORATORY MEDICINE
2015年
4期
359-362
,共4页
方健源%谢碧霞%罗韬%胡淑芬%谭冬梅
方健源%謝碧霞%囉韜%鬍淑芬%譚鼕梅
방건원%사벽하%라도%호숙분%담동매
流式细胞术%白血病细胞%外周血%复发
流式細胞術%白血病細胞%外週血%複髮
류식세포술%백혈병세포%외주혈%복발
Flow cytometry%Leukemic cell%Peripheral blood%Relapse
目的:采用多参数流式细胞术(FCM)检测急性白血病(AL)患者外周血白血病细胞的数量,为AL的临床疗效及预后提供参考。方法采用FCM检测71例AL患者外周血标本,同时检测骨髓细胞形态学的变化。结果 FCM检测外周血微小残留病变( MRD)对提示急性髓细胞白血病( AML)复发的敏感性和特异性分别是96.6%和33.3%,对提示急性淋巴细胞白血病( ALL)复发的敏感性和特异性分别是88.9%和44.4%;12例外周血MRD阴性组中有3例(25.0%)患者复发,59例外周血MRD阳性组中有44例(74.6%)复发, MRD阳性组复发率明显高于MRD阴性组( P<0.05);高MRD水平(≥10-2)组和低MRD水平(<10-2)组复发例数分别是37例(97.4%)和10例(47.6%),高MRD水平组复发率明显高于低MRD水平组(P<0.05)。结论 FCM检测外周血白血病细胞对诊断AL复发及指导个体化治疗有重要意义。
目的:採用多參數流式細胞術(FCM)檢測急性白血病(AL)患者外週血白血病細胞的數量,為AL的臨床療效及預後提供參攷。方法採用FCM檢測71例AL患者外週血標本,同時檢測骨髓細胞形態學的變化。結果 FCM檢測外週血微小殘留病變( MRD)對提示急性髓細胞白血病( AML)複髮的敏感性和特異性分彆是96.6%和33.3%,對提示急性淋巴細胞白血病( ALL)複髮的敏感性和特異性分彆是88.9%和44.4%;12例外週血MRD陰性組中有3例(25.0%)患者複髮,59例外週血MRD暘性組中有44例(74.6%)複髮, MRD暘性組複髮率明顯高于MRD陰性組( P<0.05);高MRD水平(≥10-2)組和低MRD水平(<10-2)組複髮例數分彆是37例(97.4%)和10例(47.6%),高MRD水平組複髮率明顯高于低MRD水平組(P<0.05)。結論 FCM檢測外週血白血病細胞對診斷AL複髮及指導箇體化治療有重要意義。
목적:채용다삼수류식세포술(FCM)검측급성백혈병(AL)환자외주혈백혈병세포적수량,위AL적림상료효급예후제공삼고。방법채용FCM검측71례AL환자외주혈표본,동시검측골수세포형태학적변화。결과 FCM검측외주혈미소잔류병변( MRD)대제시급성수세포백혈병( AML)복발적민감성화특이성분별시96.6%화33.3%,대제시급성림파세포백혈병( ALL)복발적민감성화특이성분별시88.9%화44.4%;12예외주혈MRD음성조중유3례(25.0%)환자복발,59예외주혈MRD양성조중유44례(74.6%)복발, MRD양성조복발솔명현고우MRD음성조( P<0.05);고MRD수평(≥10-2)조화저MRD수평(<10-2)조복발례수분별시37례(97.4%)화10례(47.6%),고MRD수평조복발솔명현고우저MRD수평조(P<0.05)。결론 FCM검측외주혈백혈병세포대진단AL복발급지도개체화치료유중요의의。
Objective To investigate the amounts of peripheral blood leukemic cells from acute leukemia ( AL ) patients determined by flow cytometry (FCM), in order to provide the reference for clinical efficiency and prognosis . Methods A total of 71 patients with AL were enrolled , and peripheral blood leukemic cells were determined by FCM . The morphological analysis of bone marrow cells was performed .Results The sensitivity and specificity for the diagnosis of acute myeloid leukemia ( AML) relapse by peripheral blood minimal residual disease ( MRD) determined by FCM were 96.6%and 33.3%, and those for the diagnosis of acute lymphocytic leukemia (ALL) relapse were 88.9%and 44.4%.In peripheral blood MRD negative group, there were 3 relapse cases of 12 patients(25.0%), and in peripheral blood MRD positive group , there were 44 relapse cases of 59 patients ( 74.6%).The relapse rate of peripheral blood MRD positive group was significantly higher than that of MRD negative group (P<0.05).The relapse amounts of high MRD level group (≥10 -2) and low MRD level group (<10 -2) were 37 cases(97.4%)and 10 cases (47.6%), and the relapse rate of high MRD level group was significantly higher than that of low MRD level group (P<0.05).Conclusions The peripheral blood leukemic cells determined by FCM has significance for the diagnosis of AL relapse and its treatment .