黑龙江医药
黑龍江醫藥
흑룡강의약
HEILONGJIANG MEDICAL JOURNAL
2015年
2期
248-250,251
,共4页
介孔碳%PEG-PLGA%分散性%给药系统
介孔碳%PEG-PLGA%分散性%給藥繫統
개공탄%PEG-PLGA%분산성%급약계통
mesoporous carbon%PEG-PLGA%dispersity%drug delivery system
目的：利用一种简便易行的方法改善介孔碳（MCN）的分散性，并以其为载体构建纳米给药系统，探索其生物应用的效果。方法：利用TEM观察聚乙二醇-聚乳酸-羟基乙酸共聚物（PEG-PLGA）包裹前后MCN的大小和形态变化。稳定性检测用于评估PEG-PLGA包裹的MCN纳米粒（MCNP）的分散性。热效应实验评价MCNP的近红外（NIR）光热转化能力。构建PEG-PLGA包裹的载阿霉素（DOX）的介孔碳给药系统（MCNPD），从Zeta电荷、载药量、释放以及细胞摄取实验对其进行表征。结果：溶液静置24h后，原始MCN几乎完全聚集沉降，而MCNP仍均匀分散；NIR照射后，MCNP表现出很好的热效应；构建的给药系统MCNPD具有较高的载药量，并且呈pH-依赖释放和NIR-触发释放，同时能够很好地摄取进入细胞。结论：PEG-PLGA包裹以提高MCN分散性的方法简单可行，成功构建了一种具有良好分散性的MCN递药载体，利于生物应用。
目的：利用一種簡便易行的方法改善介孔碳（MCN）的分散性，併以其為載體構建納米給藥繫統，探索其生物應用的效果。方法：利用TEM觀察聚乙二醇-聚乳痠-羥基乙痠共聚物（PEG-PLGA）包裹前後MCN的大小和形態變化。穩定性檢測用于評估PEG-PLGA包裹的MCN納米粒（MCNP）的分散性。熱效應實驗評價MCNP的近紅外（NIR）光熱轉化能力。構建PEG-PLGA包裹的載阿黴素（DOX）的介孔碳給藥繫統（MCNPD），從Zeta電荷、載藥量、釋放以及細胞攝取實驗對其進行錶徵。結果：溶液靜置24h後，原始MCN幾乎完全聚集沉降，而MCNP仍均勻分散；NIR照射後，MCNP錶現齣很好的熱效應；構建的給藥繫統MCNPD具有較高的載藥量，併且呈pH-依賴釋放和NIR-觸髮釋放，同時能夠很好地攝取進入細胞。結論：PEG-PLGA包裹以提高MCN分散性的方法簡單可行，成功構建瞭一種具有良好分散性的MCN遞藥載體，利于生物應用。
목적：이용일충간편역행적방법개선개공탄（MCN）적분산성，병이기위재체구건납미급약계통，탐색기생물응용적효과。방법：이용TEM관찰취을이순-취유산-간기을산공취물（PEG-PLGA）포과전후MCN적대소화형태변화。은정성검측용우평고PEG-PLGA포과적MCN납미립（MCNP）적분산성。열효응실험평개MCNP적근홍외（NIR）광열전화능력。구건PEG-PLGA포과적재아매소（DOX）적개공탄급약계통（MCNPD），종Zeta전하、재약량、석방이급세포섭취실험대기진행표정。결과：용액정치24h후，원시MCN궤호완전취집침강，이MCNP잉균균분산；NIR조사후，MCNP표현출흔호적열효응；구건적급약계통MCNPD구유교고적재약량，병차정pH-의뢰석방화NIR-촉발석방，동시능구흔호지섭취진입세포。결론：PEG-PLGA포과이제고MCN분산성적방법간단가행，성공구건료일충구유량호분산성적MCN체약재체，리우생물응용。
Objective:To improve the dispersity of mesoporous carbon nanoparticles(MCN)using asimple and practical method, and further construct a novel drug delivery system to make MCN a desirable carrier for biological application. Methods:TEM was em-ployed to observe the size and morphology of PEG-PLGA coated MCN nanoparticles (MCNP).The change of dispersitywas evaluated by stability tests. Photothermal heating effect experiment was utilized to evaluate photothermal conversion performance.MCNP-based-doxolubicin (DOX)-loaded system (MCNPD) wascharacterized by Zeta potential, drug loading content, in vitro release and cellular up-take experiments. Results:Compared to aggregation of MCN, MCNPexhibited moreuniform dispersityafter standing for 24h. And also, MCNP showed good photothermal conversion efficiencywith NIR irradiation for 5min. The drug delivery system MCNPD presented ex-cellent drug loading capacity, performed pH-responsive and NIR-triggered drug release mechanism, and showed obvious cellular up-take. Conclusion:The method that PEG-PLGA coating to improve the dispersity of MCN was simple and feasible. Anexcellent dis-persiveMCN drug delivery nanocarrierpossessing the potential of chemo-photothermal therapywas successfully obtained for biological application.
