黑龙江医药
黑龍江醫藥
흑룡강의약
HEILONGJIANG MEDICAL JOURNAL
2015年
2期
271-274
,共4页
白藜芦醇%前体脂质体%长循环热敏脂质体
白藜蘆醇%前體脂質體%長循環熱敏脂質體
백려호순%전체지질체%장순배열민지질체
resveratrol%liposomes thermosensitive proliposomes%long-circulating
目的：对白藜芦醇长循环热敏前体脂质体制备的研究以及对其性质进行分析。方法：先用薄膜分散法制备白藜芦醇长循环热敏脂质体后，再用冷冻干燥法来制备白藜芦醇长循环热敏脂质体的前体。采用电势测定仪、HPLC等方法对该脂质体的包封率、粒径、稳定性、载药量、电位、释放度等来展开系统的检查。结果：白藜芦醇长循环热敏前体脂质体水合后形成白藜芦醇长循环热敏脂质体，粒径均值为(107.9±3.6)nm，Zeta电位的均值为(-12.2±1.6)mV，包封率可达89.4%；该脂质体在相变温度42℃下药物释放达到94%以上。结论：采用长循环热敏来制备的白藜芦醇前体脂质体含量与包封率检查方法准确、快速、简单且方法简便易行。载药量大，包封率好，工艺比较稳定。本实验可为新型白藜芦醇静脉注射用热敏脂质体的研究提供基础。
目的：對白藜蘆醇長循環熱敏前體脂質體製備的研究以及對其性質進行分析。方法：先用薄膜分散法製備白藜蘆醇長循環熱敏脂質體後，再用冷凍榦燥法來製備白藜蘆醇長循環熱敏脂質體的前體。採用電勢測定儀、HPLC等方法對該脂質體的包封率、粒徑、穩定性、載藥量、電位、釋放度等來展開繫統的檢查。結果：白藜蘆醇長循環熱敏前體脂質體水閤後形成白藜蘆醇長循環熱敏脂質體，粒徑均值為(107.9±3.6)nm，Zeta電位的均值為(-12.2±1.6)mV，包封率可達89.4%；該脂質體在相變溫度42℃下藥物釋放達到94%以上。結論：採用長循環熱敏來製備的白藜蘆醇前體脂質體含量與包封率檢查方法準確、快速、簡單且方法簡便易行。載藥量大，包封率好，工藝比較穩定。本實驗可為新型白藜蘆醇靜脈註射用熱敏脂質體的研究提供基礎。
목적：대백려호순장순배열민전체지질체제비적연구이급대기성질진행분석。방법：선용박막분산법제비백려호순장순배열민지질체후，재용냉동간조법래제비백려호순장순배열민지질체적전체。채용전세측정의、HPLC등방법대해지질체적포봉솔、립경、은정성、재약량、전위、석방도등래전개계통적검사。결과：백려호순장순배열민전체지질체수합후형성백려호순장순배열민지질체，립경균치위(107.9±3.6)nm，Zeta전위적균치위(-12.2±1.6)mV，포봉솔가체89.4%；해지질체재상변온도42℃하약물석방체도94%이상。결론：채용장순배열민래제비적백려호순전체지질체함량여포봉솔검사방법준학、쾌속、간단차방법간편역행。재약량대，포봉솔호，공예비교은정。본실험가위신형백려호순정맥주사용열민지질체적연구제공기출。
Objective: Preparation and characteristic of resveratrol long-circulating thermo-sensitive proliposomes are studied. Methods:resveratrol long-circulating thermo-sensitive liposomes by film dispersion method vacuum freeze-drying technique was se-lected to dry etoposide long-circulating proliposomes, Its shape and size distribution were detected by transmission electron Zetasizer, and the content of resveratrol was determined by HPLC, The encapsulation efficiency ( EE) and the release rate of liposome were com-puted as well. Results:The mean encapsulation efficiency of the etoposide long-circulating proliposomes was 89.4%the appearance of liposomes was whole round, distribution uniform, the mean particle size were (107.9±3.6)nm nm, and zeta potential were(-12.2± 1.6)mV. The liposome 42℃temperature under the drug release above 94%. Conclusion:The methods for determination of resvera-trol content and entrapment efficiency were simple, rapid and accurate. These studies can provide bases for further development of in-travenous injection resveratrol thermo-sensitive liposomes.The preparation technology of resveratrol long-circulating thermo-sensitive proliposomes was stable, drug loading and entrapping capacity of liposomes were well.
