CAJ | 학술논문

目的：研究抑癌基因P53在膀胱癌病变临床治疗效果。方法从2013年4月~2014年1月，选取我院的69例患膀胱癌患者癌细胞标本进行回顾性分析，采用应用免疫组织化学ABC法和微波炉抗原修复法对其进行检测。其中观察组52例患者，分别采用突变型P53蛋白和p16抑癌基因。对两种抑癌因素进行对比分析。结果突变型p53蛋白与P16蛋白总阳性分别为53.62%和43.48%。p16阳性表达在病理分级、临床分期中有显著性差异(<0.05)。而p53阳性表达在临床分期中有显著性差异(<0.05)。但中低分化膀胱癌(Ⅱ+Ⅲ)与高分化膀胱癌(I级)比较具有统计学意义(<0.05)。结论 p53基因的异常是诱导膀胱癌的病发原因，表达状况与膀胱癌生物学行为密切相关与膀胱癌发生发展更是密切相关，可作为临床判断肿瘤进展及可能预后的标准。
목적：연구억암기인P53재방광암병변림상치료효과。방법종2013년4월~2014년1월，선취아원적69례환방광암환자암세포표본진행회고성분석，채용응용면역조직화학ABC법화미파로항원수복법대기진행검측。기중관찰조52례환자，분별채용돌변형P53단백화p16억암기인。대량충억암인소진행대비분석。결과돌변형p53단백여P16단백총양성분별위53.62%화43.48%。p16양성표체재병리분급、림상분기중유현저성차이(<0.05)。이p53양성표체재림상분기중유현저성차이(<0.05)。단중저분화방광암(Ⅱ+Ⅲ)여고분화방광암(I급)비교구유통계학의의(<0.05)。결론 p53기인적이상시유도방광암적병발원인，표체상황여방광암생물학행위밀절상관여방광암발생발전경시밀절상관，가작위림상판단종류진전급가능예후적표준。
Objective To study the tumor suppressor gene P53 in bladder cancer lesions,clinical therapeutic ef ect.Methods From 2013 April to 2014 January in our hospital,select 69 patients of bladder cancer cel specimens were retrospectively analyzed to detect by immunohistochemical ABC method and microwave oven antigen repairing method.The observation group of 52 cases, respectively,using mutant P53 protein and p16 tumor suppressor gene.On two tumor suppressor factor car ies on the contrast analysis.Results The mutant p53 protein and P16 protein positive respectively 53.62%and 43.48%.The positive expression of p16 in pathological grading and clinical staging with significant dif erence ( <0.05).But the positive expression of p53 had significant dif erence in the clinical staging( <0.05).But the low dif erentiation of bladder cancer(I+I I)and high dif erentiation of bladder cancer(I)compared with statistical significance( <0.05).Conclusion p53 gene abnormality is induced bladder cancer cause of disease occur ence,development and expression of the biological behavior of bladder cancer is closely associated with bladder cancer are more closely associated,as advances in clinical judgment of tumor and possible prognostic criterion.