CAJ | 학술논문

【目的】了解鞘内注射FSTL1重组蛋白对脊髓损伤后中枢性疼痛模型大鼠痛行为学影响。【方法】成年Wistar 雌性大鼠32只，随机分为4组，每组8只，分别为：空白对照组、假手术组、模型组和鞘内注射FSTL1＋模型组（FSTL组）。分别于造模前和模型建立后的4 h、12 h、24 h、d2、d4、d6、d10、d14、d21测定各组大鼠机械痛敏（机械缩腿阈值，mechanical withdrawal threshold ，MWT）和热痛敏（热缩腿潜伏期，thermal withdrawal latency ，TWL）。【结果】大鼠MWT及TWL测定结果表明：四组大鼠实验前MWT、TWL基础值相比较无统计学差异（ P ＞0．05）。假手术组监测各时间点的MWT、TWL与实验前基础值及空白对照组比较皆无统计学差异（ P ＞0．05）；模型组与术前基础值和假手术组相比较，大鼠同侧后足的MWT 在术后4 h、12 h、24 h、d2、d4、d6、d10、d14、d21时间点均明显降低（ P ＜0．05），在术后d14降低到峰值，术后d21仍低于正常水平，大鼠同侧后足的TWL值在术后4 h、12 h、24 h、d2、d4、d6、d10、d14、d21时间点均明显缩短，术后d14缩短到最低值，术后d21仍低（ P ＜0．05）；鞘内FSTL组与模型组相比较，MWT术后4 h、12 h、24 h、d2、d4、d6、d10、d14、d21均明显增加（ P ＜0．05），TWL值明显延长（ P ＜0．05）；鞘内 FSTL组与假手术组相比较，术后4 h、12 h、24 h、d2时间点的MWT 及 TWL值无明显差异（ P ＞0．05）。术后d4、d6、d10、d14、d21时间点MWT值明显低于假手术组，TWL值较假手术组明显缩短（ P ＜0．05）。【结论】鞘内注射FSTL1重组蛋白能明显减轻脊髓损伤后中枢性疼痛模型大鼠机械痛敏和热痛敏，提示FSTL1与中枢性疼痛信息的传递关系密切相关，并在中枢性疼痛的痛觉超敏（ hyper‐algesia）的产生和维持中具有重要作用。
【목적】료해초내주사FSTL1중조단백대척수손상후중추성동통모형대서통행위학영향。【방법】성년Wistar 자성대서32지，수궤분위4조，매조8지，분별위：공백대조조、가수술조、모형조화초내주사FSTL1＋모형조（FSTL조）。분별우조모전화모형건립후적4 h、12 h、24 h、d2、d4、d6、d10、d14、d21측정각조대서궤계통민（궤계축퇴역치，mechanical withdrawal threshold ，MWT）화열통민（열축퇴잠복기，thermal withdrawal latency ，TWL）。【결과】대서MWT급TWL측정결과표명：사조대서실험전MWT、TWL기출치상비교무통계학차이（ P ＞0．05）。가수술조감측각시간점적MWT、TWL여실험전기출치급공백대조조비교개무통계학차이（ P ＞0．05）；모형조여술전기출치화가수술조상비교，대서동측후족적MWT 재술후4 h、12 h、24 h、d2、d4、d6、d10、d14、d21시간점균명현강저（ P ＜0．05），재술후d14강저도봉치，술후d21잉저우정상수평，대서동측후족적TWL치재술후4 h、12 h、24 h、d2、d4、d6、d10、d14、d21시간점균명현축단，술후d14축단도최저치，술후d21잉저（ P ＜0．05）；초내FSTL조여모형조상비교，MWT술후4 h、12 h、24 h、d2、d4、d6、d10、d14、d21균명현증가（ P ＜0．05），TWL치명현연장（ P ＜0．05）；초내 FSTL조여가수술조상비교，술후4 h、12 h、24 h、d2시간점적MWT 급 TWL치무명현차이（ P ＞0．05）。술후d4、d6、d10、d14、d21시간점MWT치명현저우가수술조，TWL치교가수술조명현축단（ P ＜0．05）。【결론】초내주사FSTL1중조단백능명현감경척수손상후중추성동통모형대서궤계통민화열통민，제시FSTL1여중추성동통신식적전체관계밀절상관，병재중추성동통적통각초민（ hyper‐algesia）적산생화유지중구유중요작용。
[Objective]To explore the effects of intrathecal administration of follistatin‐like 1 recombinant protein on central pain after spinal cord injury in rats .[Methods]A total of 32 Wistar female rats were randomly divided into control ,sham ,mod‐el and FSTL1+model groups ( n =8 each) .The parameters of mechanical withdrawal threshold (MWT ) and thermal with‐drawal latency (TWL) were measured at the time of pre‐operation and 4 h ,12 h ,24 h ,d2 ,d4 ,d6 ,d10 ,d14 ,d21 post‐opera‐tion .[Results] No significant difference existed among 4 groups in baseline MWT and baseline TWL ( P>0 .05) .Compare to control group and baseline of pre‐operation ,MWT ,TWL at all postoperative timepoints had no significant change in sham group ( P >0 .05) .Compare with sham group and baseline ,preoperative MWT of ipsilateral hind decreased significantly and TWL of ipsilateral hind significantly shortened at 4h ,12h ,24h ,d2 ,d4 ,d6 ,d10 ,d14 ,d21 post‐operation in model group ,re‐duced or shortened to a peak at d14 post‐operation and continued to decrease or shorten at d21 post‐operation ( P <0 .05) . Compared with model group ,MWT of ipsilateral hind increased significantly and TWL of ipsilateral hind significantly elongated at 4h ,12h ,24h ,d2 ,d4 ,d6 ,d10 ,d14 ,d21 post‐operation in FSTL group ( P <0 .05) .Compared with sham group ,MWT , TWL at 4h ,12h ,24h ,d2 post‐operation had no significant change in FSTL group ( P>0 .05) .Compares with sham group , MWT of ipsilateral hind decreased significantly and TWL of ipsilateral hind significantly shortened at d4 ,d6 ,d10 ,d14 ,d21 post‐operation in FSTL groups ( P<0 .05) .[Conclusion]Intrathecal administration of follistatin‐like 1 recombinant protein can re‐duce mechanical and heat hyperalgesia of central pain after spinal cord injury in rats .And follistatin‐like 1 may play an impor‐tant role in nociceptive transmission of spinal level and generation and maintenance of central pain hyperalgesia .