CAJ | 학술논문

为明确山东地区葡萄霜霉病菌株的致病性及亲缘关系，本研究以不同抗性的6个葡萄品种作为鉴别寄主，采用叶盘接种法，对采自山东地区的13个葡萄霜霉病菌株进行致病性测定，并扩增每个菌株的rD-NA－ITS序列进行同源性分析。结果表明，这13个葡萄霜霉病菌株均有致病性，据致病力分为强、中、弱3种致病类型，中等致病型菌株占优势，菌株间致病力差异与菌株的地域来源无明显相关性。序列比对及系统发育树分析表明，这13个葡萄霜霉病菌株的亲缘关系很近，rDNA－ITS序列同源性高达99．94％，菌株间致病力差异与rDNA－ITS序列多态性不相关。
위명학산동지구포도상매병균주적치병성급친연관계，본연구이불동항성적6개포도품충작위감별기주，채용협반접충법，대채자산동지구적13개포도상매병균주진행치병성측정，병확증매개균주적rD-NA－ITS서렬진행동원성분석。결과표명，저13개포도상매병균주균유치병성，거치병력분위강、중、약3충치병류형，중등치병형균주점우세，균주간치병력차이여균주적지역래원무명현상관성。서렬비대급계통발육수분석표명，저13개포도상매병균주적친연관계흔근，rDNA－ITS서렬동원성고체99．94％，균주간치병력차이여rDNA－ITS서렬다태성불상관。
In order to study the virulence differentiation and genetic relationship of Plasmopara viticola in Shandong,13 different strains of P.viticola were collected from different regions in Shandong Province.Their pathogenicity were tested through infecting 6 grape varieties with different resistance by inoculating leaf disc method,and their homology were analyzed by amplifying the rDNA -ITS sequences with primers ITS1F/ITS4R.The results indicated that all the 13 strains had pathogenicity.The cluster analysis concluded that they were roughly divided into weak, moderate and strong pathotypes, and the moderate pathotype was dominant. The pathogenicity differentiation was not obviously correlated with the regional origin.The sequence alignment and phylogenetic tree analysis results showed that there were very close genetic relationships between the 13 strains with the homolygy of rDNA-ITS sequences as 99.94%;moreover,there was no correlation between virulence differentiation and rDAN-ITS sequences polymorphism.