CAJ | 학술논문

为了明确苎麻不同 SSR 的遗传差异性，利用 Genomic －SSR 与 EST －SSR 两种 SSR标记对19个苎麻核心种质的遗传多样性进行分析。根据统计结果，Genomic －SSR 平均检测到的观测等位基因数为3.4643、有效等位基因数为2.1982、Shannon 多样性指数为0.8864、观测杂合度为0.5254、期望杂合度为0.5172；EST －SSR 平均检测到的观测等位基因数为2.3333、有效等位基因数为1.9438、Shannon 多样性指数为0.7120、观测杂合度为0.5128、期望杂合度为0.4778。Genomic －SSR 和 EST －SSR 两种引物的有效等位基因数（Ne）和观测杂合度（Ho）差异不显著，Genomic －SSR 的 Shannon 指数显著高于 EST －SSR。研究结果表明，在标记多态性及基因型鉴别等方面，Genomic －SSR 与 EST －SSR 均具有显著的遗传差异性，但是两种标记都能有效的鉴别不同基因型个体。Genomic －SSR 可优先用于分子身份证、高密度遗传连锁图谱的构建和遗传多样性分析；EST －SSR 用来研究苎麻近缘种间的遗传多样性、进化分析、连锁图谱和比较基因组学更有优越性。
위료명학저마불동 SSR 적유전차이성，이용 Genomic －SSR 여 EST －SSR 량충 SSR표기대19개저마핵심충질적유전다양성진행분석。근거통계결과，Genomic －SSR 평균검측도적관측등위기인수위3.4643、유효등위기인수위2.1982、Shannon 다양성지수위0.8864、관측잡합도위0.5254、기망잡합도위0.5172；EST －SSR 평균검측도적관측등위기인수위2.3333、유효등위기인수위1.9438、Shannon 다양성지수위0.7120、관측잡합도위0.5128、기망잡합도위0.4778。Genomic －SSR 화 EST －SSR 량충인물적유효등위기인수（Ne）화관측잡합도（Ho）차이불현저，Genomic －SSR 적 Shannon 지수현저고우 EST －SSR。연구결과표명，재표기다태성급기인형감별등방면，Genomic －SSR 여 EST －SSR 균구유현저적유전차이성，단시량충표기도능유효적감별불동기인형개체。Genomic －SSR 가우선용우분자신빈증、고밀도유전련쇄도보적구건화유전다양성분석；EST －SSR 용래연구저마근연충간적유전다양성、진화분석、련쇄도보화비교기인조학경유우월성。
In order to clarify the genetic differences among different SSRs,Genomic -SSR and EST-SSR were used to analyze the genetic diversity of 19 important ramie genetic resources.According to the statistical results,the average observed -alleles detected by Genomic -SSR was 3.4643,effective alleles was 2.1982,shannon was 0.8864,heterozygosity was 0.5254,expected heterozygosity was 0.5172.The average observed -alleles detected by EST -SSR was 2.3333,effective alleles was 1.9438,shannon was 0.7120,heterozygosity was 0.5128,expected heterozygosity was 0.4778.There were no significant differences in effective number of alleles (Ne)and observed heterozygosity (Ho)be-tween Genomic -SSR and EST -SSR.Genomic -SSR Shannon index was significantly higher than that of EST -SSR.The above results showed there were significant genetic differences in marker polymor-phism and genotype identification between Genomic -SSR and EST -SSR,but the two kinds of markers could also effectively identify different genotypes.Genomic -SSR had priority for molecular identifica-tion,construction of high -density genetic linkage map and analysis of genetic diversity,EST -SSR had more advantages of studying the genetic diversity among close ramie species,evolutionary analysis,ge-netic map and comparative genomics.