CAJ | 학술논문

目的研究蛋白酶体核心亚基在人动脉粥样硬化斑块中的表达水平,探讨蛋白酶体组成及功能与动脉粥样斑块病变间的关系. 方法搜集颈动脉狭窄并行颈动脉内膜切除术患者病例组和主动脉夹层并行主动脉置换术患者对照组的相关临床资料.选择病例组中5例术中所切除的颈动脉粥样硬化斑块和斑块旁内膜组织,对照组中5例术中所切除的相对正常的锁骨下动脉和无名动脉组织,采用免疫印迹(Western)法检测并比较其中的蛋白酶体核心亚基的蛋白质水平. 结果病例组高脂血症、糖尿病及吸烟比率与对照组比较差异无统计学意义(x2值分别为0.61、1.67、1.67,均 P＞0.05);与对照组比较,β类亚基中组成型亚基β3、β4(F值分别为56.576、27.846,均P＜0.05)以及催化亚基β1和β5在斑块核心部分的表达水平下调(F值分别为8.221、82.716,均P＜0.05),而免疫亚基β1i和β5i的表达水平升高(F值分别为50.167、229.743,均P＜0.05). 结论免疫蛋白酶体特征性催化亚基β1i和β5i在粥样斑块核心组织中表达上调,提示由组成型蛋白酶体(β1和β5为其特征性催化亚基)向免疫蛋白酶体转变这一动态过程与动脉粥样硬化斑块形成过程密切相关.
목적 연구단백매체핵심아기재인동맥죽양경화반괴중적표체수평,탐토단백매체조성급공능여동맥죽양반괴병변간적관계. 방법 수집경동맥협착병행경동맥내막절제술환자병례조화주동맥협층병행주동맥치환술환자대조조적상관림상자료.선택병례조중5례술중소절제적경동맥죽양경화반괴화반괴방내막조직,대조조중5례술중소절제적상대정상적쇄골하동맥화무명동맥조직,채용면역인적(Western)법검측병비교기중적단백매체핵심아기적단백질수평. 결과 병례조고지혈증、당뇨병급흡연비솔여대조조비교차이무통계학의의(x2치분별위0.61、1.67、1.67,균 P＞0.05);여대조조비교,β류아기중조성형아기β3、β4(F치분별위56.576、27.846,균P＜0.05)이급최화아기β1화β5재반괴핵심부분적표체수평하조(F치분별위8.221、82.716,균P＜0.05),이면역아기β1i화β5i적표체수평승고(F치분별위50.167、229.743,균P＜0.05). 결론 면역단백매체특정성최화아기β1i화β5i재죽양반괴핵심조직중표체상조,제시유조성형단백매체(β1화β5위기특정성최화아기)향면역단백매체전변저일동태과정여동맥죽양경화반괴형성과정밀절상관.
Objective To explore the relationship of atherosclerotic plaques with the component and function of proteasome complex by systematically investigating the protein levels of proteasome core subunits in human atherosclerotic plaques.Methods Clinical data of the observation group (patients with carotid artery stenosis undergoing carotid artery endarterectomy) and control group (patients with aortic dissection who underwent aortic replacement) were collected.The carotid endarterectomy specimens including the carotid atherosclerosis plaques and endometrial tissues adjacent to plaques were removed from 5 patients in observation group,and the relatively normal arterial intima(subclavian artery and innominate artery) were taken from 5 patients in control group.The protein levels of the proteasome subunits were measured by Western blotting.Results There was no significant difference in the occurrences of diabetes,hyperlipidemia and smoking between the two groups (x2 =0.61,1.67 and 1.67,all P＞ 0.05).Compared with the control group,the observation group showed that the levels of two non-catalytic β subunits β3 and β4 (F=56.576 and 27.846,both P＜0.05),and the catalytic subunits β1 and β5 (F=8.221 and 82.716,both P＜0.05) were significantly down-regulated in the plaques,whereas the expressions of β1i and β5i in the plaques (F=50.167、229.743,P＜0.05) were robustly elevated.Conclusions The levels of the catalytic subunits of immune proteasome β1i and β5i are up-regulated in the atherosclerotic plaques,which indicates that the transformation from the constitutive proteasomes (β1 and β5) to the immune-proteasomes (β1i and β5i) is highly involved in the atherosclerosis.