CAJ | 학술논문

目的：探讨痰瘀阻络模型大鼠的血液流变学和红细胞膜的生物学特征变化。方法：选用SD雄性大鼠40只，按体质量随机分为四组，每组10只。10天组分为空白组和痰瘀阻络模型组两组；20天组同样分为空白组和模型组两组。模型组分别采用脂乳剂连续灌胃10天和20天复制高脂血症模型，取血测定血液流变学和红细胞膜组分相关指标。结果：与空白组比较，痰瘀阻络模型组大鼠血浆总胆固醇（CHO）﹑甘油三酯（TG）﹑低密度脂蛋白胆固醇（LDL－C）﹑血糖（GLU）含量均显著升高（P＜0．05），高密度脂蛋白胆固醇（HDL－C）含量显著降低（P＜0．05）；全血黏度﹑血浆黏度均显著升高（P＜0．05， P＜0．01）；Na＋－K＋－ATP酶活力﹑SOD活力均不同程度降低（P＜0．05，P＜0．05），MDA含量显著升高（P＜0．05），唾液酸含量有降低趋势，但无显著性差异；膜胆固醇含量极显著升高（P＜0．01）。结论：痰瘀阻络模型中胆固醇含量的升高，引起血浆黏度的增大，可能导致红细胞膜上SOD酶活力、Na＋－K＋－ATP酶活力降低，MDA含量升高，膜胆固醇含量显著升高，同时唾液酸含量也有降低趋势。
목적：탐토담어조락모형대서적혈액류변학화홍세포막적생물학특정변화。방법：선용SD웅성대서40지，안체질량수궤분위사조，매조10지。10천조분위공백조화담어조락모형조량조；20천조동양분위공백조화모형조량조。모형조분별채용지유제련속관위10천화20천복제고지혈증모형，취혈측정혈액류변학화홍세포막조분상관지표。결과：여공백조비교，담어조락모형조대서혈장총담고순（CHO）﹑감유삼지（TG）﹑저밀도지단백담고순（LDL－C）﹑혈당（GLU）함량균현저승고（P＜0．05），고밀도지단백담고순（HDL－C）함량현저강저（P＜0．05）；전혈점도﹑혈장점도균현저승고（P＜0．05， P＜0．01）；Na＋－K＋－ATP매활력﹑SOD활력균불동정도강저（P＜0．05，P＜0．05），MDA함량현저승고（P＜0．05），타액산함량유강저추세，단무현저성차이；막담고순함량겁현저승고（P＜0．01）。결론：담어조락모형중담고순함량적승고，인기혈장점도적증대，가능도치홍세포막상SOD매활력、Na＋－K＋－ATP매활력강저，MDA함량승고，막담고순함량현저승고，동시타액산함량야유강저추세。
Objective:To investigate the changes of biological characteristics of hemorheology and erythrocyte membrane of model rats with stagnation of phlegm and blood.Methods:40 SD male rats were randomly divided into four groups, with ten in each group.Ten-days group was subdivided into a blank group and a model group;twenty-days group was also subdivided into a blank group and a model group.High fat emulsion was continuously given to model rats intragas-trically for 10 days and 20 days respectively, and then determine the hemorheology indexes and activity of erythrocyte membrane.Results:Compared with the blank group, the serous CHO, TG, LDL-C and GLU were increased signifi-cantly (P<0.05), and HDL-C was decreased obviously (P<0.05); the whole blood viscosity and the plasma vis-cosity were increased obviously ( P<0 .05 , P<0 .01 );Na +-K+-ATP enzyme and SOD activity were decreased ob-viously(P<0.05, P<0.05), and the contents of MDA and membrane cholesterol were increased significantly (P<0.05), and SA had a decreased tendency.Conclusion:Models with stagnation of phlegm and blood can cause the in-crease of cholesterol, and the plasma viscosity is also increased.It might lead to the decrease of SOD and Na +-K+-ATP enzyme, meanwhile, MDA and membrane cholesterol contents have been changed to different degrees.Meanwhile, the content of SA has a decreased tendency.