CAJ | 학술논문

目的：研究和探讨天花粉水煎剂在体外对人黑色素瘤A375细胞的形态、增殖、周期和迁移的影响。方法：用显微镜观察天花粉水煎剂作用前后的A375细胞形态的改变；利用MTT法检测天花粉水煎剂对A375细胞生长和增殖的影响；采用流式细胞术检测天花粉水煎剂对A375细胞周期的影响；利用划痕实验观察天花粉水煎剂对A375细胞迁移的影响。结果：显微镜观察结果和MTT结果显示，利用不同质量浓度的天花粉水煎剂处理后，正常形态的细胞明显减少，细胞死亡数量增加；而且对A375细胞的增殖有不同程度的抑制，抑制率呈质量浓度依赖性。流式细胞术分析结果发现，随着质量浓度的增加，细胞增殖周期中G2期中细胞所占百分数与对照组相比，有统计学差异；细胞划痕实验结果显示，640和2560μg／mL天花粉水煎剂组划痕愈合率低。结论：天花粉水煎剂在体外可以改变A375细胞的正常形态，导致细胞死亡；对A375细胞的增殖周期具有抑制作用，同时诱导细胞周期中的G2期阻滞；一定浓度的天花粉水煎剂对A375细胞的迁移具有抑制作用，可以有效地抑制黑色素瘤细胞的恶化和转移，有利于黑色素瘤的预后。
목적：연구화탐토천화분수전제재체외대인흑색소류A375세포적형태、증식、주기화천이적영향。방법：용현미경관찰천화분수전제작용전후적A375세포형태적개변；이용MTT법검측천화분수전제대A375세포생장화증식적영향；채용류식세포술검측천화분수전제대A375세포주기적영향；이용화흔실험관찰천화분수전제대A375세포천이적영향。결과：현미경관찰결과화MTT결과현시，이용불동질량농도적천화분수전제처리후，정상형태적세포명현감소，세포사망수량증가；이차대A375세포적증식유불동정도적억제，억제솔정질량농도의뢰성。류식세포술분석결과발현，수착질량농도적증가，세포증식주기중G2기중세포소점백분수여대조조상비，유통계학차이；세포화흔실험결과현시，640화2560μg／mL천화분수전제조화흔유합솔저。결론：천화분수전제재체외가이개변A375세포적정상형태，도치세포사망；대A375세포적증식주기구유억제작용，동시유도세포주기중적G2기조체；일정농도적천화분수전제대A375세포적천이구유억제작용，가이유효지억제흑색소류세포적악화화전이，유리우흑색소류적예후。
Objective:To investigate the effect of Radix Trichosanthis decoction on cell morphology, cell proliferation and cell migration in human melanoma A375 cells in vitro.Methods:Use the microscope and MTT method and the changes of the cellular morphology and cell proliferation were detected in A375 cells treated by Radix Trichosanthis decoction;flo wing cytometry was applied to detect the cell cycle in A375 cells;cell migration was evaluated by using scratch experi-ment and the effect of the cell migration was assessed by the scarification test.Results: Compared with the control group, we found that A375 cells treated by Radix Trichosanthis decoction had the lowest cell viability and the number of normal morphology cells was significantly reduced in A375 cells in concentration-dependent manner.A375 cells trea-ted with Radix Trichosanthis decoction showed that cell proliferation was more obviously suppressed than those untreated control using MTT assay.Flowing cytometry assay showed the percentage of G2 phase cells had a significant difference compared with the control group treated by different concentration Radix Trichosanthis decoction.The cell scratch analy-sis indicated A375 cells treated by 640 and 2 560μg/mL Radix Trichosanthis decoction had lower wound healing rate. Conclusion:We found Radix Trichosanthis decoction could cause cell death and cell morphology abnormal in vitro.We also found Radix Trichosanthis decoction could inhibit cell proliferation and cell migration in concentration-dependent manner.Interestingly, G2 phase arrest could be induced by Radix Trichosanthis decoction.Our findings can provide new knowledge about Radix Trichosanthis decoction in development of melanoma and indicate the potential application of Radix Trichosanthis decoction in the prognosis of melanoma.