CAJ | 학술논문

目的：对半夏中分离的一株内生真菌BX－9（青霉属）次级代谢产物进行分离鉴定及生物活性研究。方法：将一株半夏内生真菌（ BX－9）用PDB培养基进行发酵培养，菌丝体用甲醇回流提取2次，菌液用乙酸乙酯萃取两次，通过各种柱色谱分离得到次级代谢化合物。采用MTT法和改良的Ellman法测定化合物抗肿瘤和抗乙酰胆碱酯酶活性。结果：分离得到7个化合物，分别为2－Hydroxy－14－tricosenvic acid（1）、4，6，8，22－tetraen－3－one－ergostane（2）、Ergosterol（3）、Ergosterol peroxide（4）、（＋）－Brefeldin A （5）、7－oxobrefeldin A （6）、4－epi－Brefeldin A （7）。化合物3～6对Hela、7721、HL60三株肿瘤细胞均有显著活性。这7个化合物均无乙酰胆碱酯酶抑制活性。结论：从半夏内生菌BX－9中能分离出具有抗肿瘤活性的次生代谢产物，其中Brefeldin A含量较高。
목적：대반하중분리적일주내생진균BX－9（청매속）차급대사산물진행분리감정급생물활성연구。방법：장일주반하내생진균（ BX－9）용PDB배양기진행발효배양，균사체용갑순회류제취2차，균액용을산을지췌취량차，통과각충주색보분리득도차급대사화합물。채용MTT법화개량적Ellman법측정화합물항종류화항을선담감지매활성。결과：분리득도7개화합물，분별위2－Hydroxy－14－tricosenvic acid（1）、4，6，8，22－tetraen－3－one－ergostane（2）、Ergosterol（3）、Ergosterol peroxide（4）、（＋）－Brefeldin A （5）、7－oxobrefeldin A （6）、4－epi－Brefeldin A （7）。화합물3～6대Hela、7721、HL60삼주종류세포균유현저활성。저7개화합물균무을선담감지매억제활성。결론：종반하내생균BX－9중능분리출구유항종류활성적차생대사산물，기중Brefeldin A함량교고。
Objective:To isolate the secondary metabolites from the fermentation broth and mycelium of an endophytic fun-gi BX-9 ( Penicillium sp.) isolated from Pinellia ternate ( Thunb.) and detect the biological activity of these secondary metabolites.Methods:The strain BX-9 was fermented in PDB culture,and fermentation broth was extracted by ethyl acetate,and the mycelia of endophytic were extracted in methanol for two times.The chemical constituents of the strain BX-9 were isolated by variety of chromatographyic methods.The AChE inhibitory and antitumor activity was deter-mined by modified Ellman’ s method and MTT method.Results:Seven compounds were isolated from the fermentation broth and mycelium of endophytic fungi from Pinellia ternate and they were identified as 2-Hydroxy-14-tricosenvic acid (1), [4, 6, 8, 22-tetraen-3-one-ergostane] (2),Ergosterol (3),Ergosterol peroxide (4),( +) -Brefel-din A (5), 7-oxobrefeldin A (6), 4-epi-Brefeldin A (7).All of the compounds had no AChE inhibitory activity. Compounds (3~6) had a remarkably antitumor activity against Hela,7721, HL60.Conclusion:It is possible to isolate antitumor compounds from endophytic fungi of Pinellia ternate and the strain BX-9 can produce large numbers of brefel-din A.