CAJ | 학술논문

目的：建立测定连翘不同部位中松脂醇β－D葡萄糖苷、表松脂醇β－D葡萄糖苷、连翘苷、连翘脂素和连翘酯苷5种成分的高效液相荧光定量方法（ HPLC －FLD ）。方法：采用色谱柱 Dima C18（4.6mm ×250mm，5μm），流动相甲醇（A）－水（B）梯度洗脱系统，流速1．0mL／min。木脂素类成分荧光检测激发波长为270nm，发射波长为340nm；咖啡酸苯乙醇苷类成分激发波长为320nm，发射波长为460 nm。结果：5种成分均达到基线分离，各成分均有较宽的线性范围和良好的线性关系（ r＞0．998）；回收率在95．68％～102．59％。结论：连翘不同部位中5种成分的定性定量测定方法准确、灵敏、实用，为连翘的质量评价提供理论依据。
목적：건립측정련교불동부위중송지순β－D포도당감、표송지순β－D포도당감、련교감、련교지소화련교지감5충성분적고효액상형광정량방법（ HPLC －FLD ）。방법：채용색보주 Dima C18（4.6mm ×250mm，5μm），류동상갑순（A）－수（B）제도세탈계통，류속1．0mL／min。목지소류성분형광검측격발파장위270nm，발사파장위340nm；가배산분을순감류성분격발파장위320nm，발사파장위460 nm。결과：5충성분균체도기선분리，각성분균유교관적선성범위화량호적선성관계（ r＞0．998）；회수솔재95．68％～102．59％。결론：련교불동부위중5충성분적정성정량측정방법준학、령민、실용，위련교적질량평개제공이론의거。
Objective:To establish an high performance liquid chromatography and fluorescence detection ( HPLC-FLD) method for simultaneous determination of five components such as (+)-pinoresinol-β-D-glucoside, (+)-epipi-noresinol-β-D-glucoside, phillyrin, phillygenin and forsythoside in different parts of Fosrythia suspensa.Methods:The separation was carried out in a reversed phase C 18 column (4.6mm ×250mm ,5μm) by a gradient elution program with a mobile phase gradient consisting of methanol and water at the flow rate of 1.0 mL/min.The lignan profile was re-corded at λex/λem =270/340nm, while the caffeoyl phenylethanoid glycosides profile was recorded at λex/λem =320/460nm.Results:The five components were separated to baseline.Each component had a wide linear range and a good linear relationship (r>0.998);recoveries were from 95.68%to 102.59%.Conclusion:The qualitative and quantita-tive analysis of F.suspensa samples by using HPLC-FLD method is accurate, sensitive and practical ,which provides a scientific basis for the quality evaluation of F.suspensa.