CAJ | 학술논문

目的：建立瓜蒌皮中游离氨基酸类成分指纹图谱，并比较研究不同品种、产地瓜蒌皮氨基酸含量差异，为科学评价与有效控制瓜蒌皮质量提供依据。方法：采用异硫氰酸苯酯（ PITC）为衍生试剂，色谱柱为Eclipse XDB C18（4．6mm ×250mm，5μm）；流动相A：乙腈－水（4∶1），流动相B：醋酸钠－乙腈溶液（93∶7），梯度洗脱；流速：1mL／min；检测波长：254nm；柱温为30℃。结果：建立了瓜蒌皮中19种游离氨基酸类成分液相指纹图谱共有模式，并对不同产地瓜蒌皮药材进行了相似度比较和聚类分析。结论：本方法简便、快速、重复性好，可用于鉴别和区分不同来源的瓜蒌皮药材，为全面控制瓜蒌皮药材的质量提供依据。
목적：건립과루피중유리안기산류성분지문도보，병비교연구불동품충、산지과루피안기산함량차이，위과학평개여유효공제과루피질량제공의거。방법：채용이류청산분지（ PITC）위연생시제，색보주위Eclipse XDB C18（4．6mm ×250mm，5μm）；류동상A：을정－수（4∶1），류동상B：작산납－을정용액（93∶7），제도세탈；류속：1mL／min；검측파장：254nm；주온위30℃。결과：건립료과루피중19충유리안기산류성분액상지문도보공유모식，병대불동산지과루피약재진행료상사도비교화취류분석。결론：본방법간편、쾌속、중복성호，가용우감별화구분불동래원적과루피약재，위전면공제과루피약재적질량제공의거。
Objective:To set up fingerprints of amino acid of trichosanthes pericarpium from different varieties and habitats by HPLC,in order to establish a sensitive and specific method for controlling the quality of trichosanthes pericarpium. Methods:Phenyl isothiocyanate ( PITC) was used as a derivatization reagent.Chromatographic condition:Eclipse XDB C18 of HPLC column, (4.6mm ×250mm,5μm);mobile phase:gradient elution of acetonitrile-water (4∶1) (A) and sodium acetate solution-acetonitrile (93∶7) (B), detector wavelength:254nm;column temperature:30℃at the flow rate of 1mL/min.Results:The common modes of HPLC fingerprints of 19 kinds of amino acid in trichosanthes pericarpi-um were set up and the similar degrees to the crude drugs of different varieties and habitats were compared .Conclusion:The method is accurate, reliable and sensitive, which can be used as a quality control item for trichosanthes pericarpium collected from different sources and varieties.