CAJ | 학술논문

目的：基于Hertz弹性接触模型，基于原子力显微镜（ AFM）研究姜黄素对肺癌细胞的机械特性尤其是弹性模量的影响。方法：利用核小体酶联免疫吸附试验检测姜黄素作用后的细胞凋亡，利用AFM检测癌细胞的表面形貌和机械特性。结果：姜黄素明显诱导癌细胞产生凋亡，凋亡细胞的弹性模量和硬度明显增加。结论：姜黄素诱导细胞凋亡的机制提示细胞骨架的参与，癌细胞的硬度增加可能进一步抑制细胞的远处迁移和运动。
목적：기우Hertz탄성접촉모형，기우원자력현미경（ AFM）연구강황소대폐암세포적궤계특성우기시탄성모량적영향。방법：이용핵소체매련면역흡부시험검측강황소작용후적세포조망，이용AFM검측암세포적표면형모화궤계특성。결과：강황소명현유도암세포산생조망，조망세포적탄성모량화경도명현증가。결론：강황소유도세포조망적궤제제시세포골가적삼여，암세포적경도증가가능진일보억제세포적원처천이화운동。
Objective:To study the effect of curcumin on mechanical properties of lung cancer cells, especially on the modulus of elasticity.Methods:Use nucleosome enzyme-linked immunosorbent assay to detect cell apoptosis after curcu-min role,and use atomic force microscope to detect the surface morphology and mechanical properties of cancer cells.Re-sults:Curcumin significantly induced apoptosis of cancer cells, and the elastic modulus and hardness of the apoptosis cells increased significantly.Conclusion:The mechanism of apoptosis induced by curcumin prompts the participation of cytoskeleton,and the increased hardness of cancer cells can further inhibit cell migration and movement in the distance.