CAJ | 학술논문

目的：检测肝癌细胞中转录因子 KLF6的表达水平，确定 KLF6对肿瘤转移相关基因 Basigin -2的转录调控机制，并探讨其对 Basigin -2介导的肝癌细胞侵袭转移的调控作用。方法：培养肝癌细胞及正常肝细胞，提取总 RNA，real - time RT - PCR 方法确定 KLF6和 Basigin -2的表达水平。荧光素酶报告基因系统检测KLF6对 Basigin -2的转录调控作用。过表达或干涉 KLF6基因，观察 Basigin -2表达水平的变化，并用 Tran-swell 方法检测 KLF6对肝癌细胞体外侵袭力的影响。结果：相对于正常肝细胞，肝癌细胞中 Basigin -2在mRNA 水平表达上调，而 KLF6表达水平下调。KLF6可以与 Basigin -2启动子区结合，抑制 Basigin -2的转录活性。肝癌细胞中过表达 KLF6可以在 mRNA 水平上抑制 Basigin -2的转录表达，而干涉 KLF6则表现为Basigin -2的表达上调。过表达 KLF6可以抑制肝癌细胞的体外侵袭力，相反干涉 KLF6促进了肝癌细胞的侵袭转移。结论：转录因子 KLF6抑制 Basigin -2的转录激活，KLF6可以抑制 Basigin -2介导的肝癌细胞的侵袭转移。
목적：검측간암세포중전록인자 KLF6적표체수평，학정 KLF6대종류전이상관기인 Basigin -2적전록조공궤제，병탐토기대 Basigin -2개도적간암세포침습전이적조공작용。방법：배양간암세포급정상간세포，제취총 RNA，real - time RT - PCR 방법학정 KLF6화 Basigin -2적표체수평。형광소매보고기인계통검측KLF6대 Basigin -2적전록조공작용。과표체혹간섭 KLF6기인，관찰 Basigin -2표체수평적변화，병용 Tran-swell 방법검측 KLF6대간암세포체외침습력적영향。결과：상대우정상간세포，간암세포중 Basigin -2재mRNA 수평표체상조，이 KLF6표체수평하조。KLF6가이여 Basigin -2계동자구결합，억제 Basigin -2적전록활성。간암세포중과표체 KLF6가이재 mRNA 수평상억제 Basigin -2적전록표체，이간섭 KLF6칙표현위Basigin -2적표체상조。과표체 KLF6가이억제간암세포적체외침습력，상반간섭 KLF6촉진료간암세포적침습전이。결론：전록인자 KLF6억제 Basigin -2적전록격활，KLF6가이억제 Basigin -2개도적간암세포적침습전이。
Objective:To explore the expression of transcription factor KLF6,determine the regulation of KLF6 on tumor metastasis related gene Basigin - 2 and observe roles of KLF6 on hepatocellular carcinoma cells invasion and metastasis. Methods:Hepatocellular carcinoma cells and normal hepatocytes were cultured. In purified total RNA,ex-pression levels of KLF6 and Basigin - 2 were detected using real - time RT - PCR. The transcriptional regulation of KLF6 on Basigin - 2 were inspected with luciferase reporter assay system. Through overexpressing or interfering KLF6,the expression regulation of KLF6 on Basigin - 2 were observed,and the role of KLF6 on hepatocellular carci-noma cells invasion was explored using in vitro transwell invasion assay. Results:Compared to normal hepatocyte con-trol,the expression levels of Basigin - 2 were obviously up - regulated in mRNA level,however those of KLF6 were down - regulated. Transcription factor KLF6 could bind to genome DNA located on the promoter region of Basigin - 2, and repress Basigin - 2 transcription. Overexpressing KLF6 in HCC cells could inhibit the expression of Basigin - 2, while knocking down KLF6 could up - regulate the expression of Basigin - 2. Overexpressed KLF6 also could inhibit hepatocellular carcinoma cells invasion and metastasis. Conclusion:Transcription factor KLF6 repressed the transcrip-tional regulation of Basigin - 2. KLF6 could inhibit Basigin - 2 mediated hepatocellular carcinoma cells invasion and metastasis.