CAJ | 학술논문

红檵木是极具观赏价值的常绿灌木。以红檵木的叶片和茎段为材料，通过优化愈伤组织诱导与增殖，不定芽诱导、增殖与生根的培养基与激素配比，进行红檵木高效再生体系研究。结果表明，红檵木外植体最佳消毒方案为两次升汞消毒法：0.1% HgCl2两次消毒，各3 min；最佳愈伤组织诱导与增殖的培养基配方分别为：MS +6-BA 1.0 mg/L + NAA 1.7 mg/L、MS +6-BA 1.0 mg/L + IBA 0.06 mg/L；首次使用 AgNO3，获得最佳不定芽诱导培养基配方：MS +6-BA 1.5 mg/L+ NAA 0.01 mg/L + AgNO31 mg/L；不定芽增殖最佳培养基配方为：MS +6-BA 1.0 mg/L + IBA 0.05 mg/L + Vc 5.0 mg/L；最佳不定芽生根方案为：选择2.1～3.0 cm 的不定芽，保留2～4叶片，接种至1/2 MS + IBA 4.5 mg/L 培养基。从而建立了效果稳定的完整红檵木高效再生体系，为红檵木工厂化育苗及转基因体系建立奠定基础。
홍계목시겁구관상개치적상록관목。이홍계목적협편화경단위재료，통과우화유상조직유도여증식，불정아유도、증식여생근적배양기여격소배비，진행홍계목고효재생체계연구。결과표명，홍계목외식체최가소독방안위량차승홍소독법：0.1% HgCl2량차소독，각3 min；최가유상조직유도여증식적배양기배방분별위：MS +6-BA 1.0 mg/L + NAA 1.7 mg/L、MS +6-BA 1.0 mg/L + IBA 0.06 mg/L；수차사용 AgNO3，획득최가불정아유도배양기배방：MS +6-BA 1.5 mg/L+ NAA 0.01 mg/L + AgNO31 mg/L；불정아증식최가배양기배방위：MS +6-BA 1.0 mg/L + IBA 0.05 mg/L + Vc 5.0 mg/L；최가불정아생근방안위：선택2.1～3.0 cm 적불정아，보류2～4협편，접충지1/2 MS + IBA 4.5 mg/L 배양기。종이건립료효과은정적완정홍계목고효재생체계，위홍계목공엄화육묘급전기인체계건립전정기출。
Loropetalum chinense var. rubrum is a kind of evergreen shrub which has great ornamental value. Using stems and leaves as explant, the high-efficient regeneration system of L. chinense var. rubrum was researched by optimizing the medium type and hormone combination in induction and multiplication of callus, induction and rooting of adventitious buds. The results show that the optimal sterilizing conditions was: disinfected twice with 0.1% HgCl2 sterilization each lasted 3 minutes; the optimal mediums for callus induction and multiplication were: MS+6-BA 1.0 mg/L+NAA1.7 mg/L and MS + 6-BA 1.0 mg/L+ IBA 0.06 ~ 0.08 mg/L, respectively; By introducing AgNO3 firstly, the optimal medium for adventitious bud induction was confirmed as: MS + 6-BA 1.5 mg/L+NAA 0.01 mg/L + AgNO3 1 mg/L; the optimal medium of adventitious bud multiplication was: MS + 6-BA 1.0 mg/L + IBA 0.05 mg/L+ Vc 5.0 mg/L. The optimal program for rooting of adventitious bud is as follows steps: choosing 2.1~3.0 cm tall buds with 2~4 leaves and inoculating to the medium 1/2 MS + IBA 4.5 mg/L. Thereby, the high-efficient regeneration system of L. chinense var. rubrum was established, and this system provide a basis for establishing the transgenic system and implementing industrialized breeding and seedling.