CAJ | 학술논문

目的：建立同时测定鱼腥草中绿原酸和金丝桃苷含量的高效液相色谱法。方法色谱柱为 Akzo Nobel Kromasil C18柱（250 mm ×4.6 mm，5μm），流动相为乙腈－0.1%磷酸溶液，梯度洗脱，检测波长326 nm。结果绿原酸和金丝桃苷进样量在0.204~2.040μg （ r =0.9999）和0.210~2.100μg（ r =0.9998）范围内与峰面积呈良好线性关系，平均回收率分别为98.46%（ RSD =1.00%）和98.37%（ RSD =0.97%）。结论该方法简便、快速、准确、重复性好，可用于鱼腥草中2种有机酸类成分的含量测定。
목적：건립동시측정어성초중록원산화금사도감함량적고효액상색보법。방법색보주위 Akzo Nobel Kromasil C18주（250 mm ×4.6 mm，5μm），류동상위을정－0.1%린산용액，제도세탈，검측파장326 nm。결과록원산화금사도감진양량재0.204~2.040μg （ r =0.9999）화0.210~2.100μg（ r =0.9998）범위내여봉면적정량호선성관계，평균회수솔분별위98.46%（ RSD =1.00%）화98.37%（ RSD =0.97%）。결론해방법간편、쾌속、준학、중복성호，가용우어성초중2충유궤산류성분적함량측정。
Objective To establish a HPLC method for the simultaneous determination of chlorogenic acid and hyperin contents in Herba Houttuyniae. Methods The separation was carried out on the Akzo Nobel Kromasil C18 column(250 mm × 4. 6 mm, 5 μm) . The mobile phase was methanol - 0. 1% phosphoric acid with gradient elution. The detective wavelength was set at 326 nm. Results Chlorogenic acid and hyperin had good linearity in the range of 0. 204 - 2. 040 μg( r = 0. 999 9) and 0. 210 - 2. 100 μg( r = 0. 999 8) respectively. The average recovery rates were 98. 46% ( RSD = 1. 00% ) and 98. 37% ( RSD = 0. 97% ) respectively. Conclusion This method is simple, rapid and accurate with good repeatability, and can be used for quantitatively determining the contents of 2 kinds of organic acid component in Herba Houttuyniae.