CAJ | 학술논문

目的：应用 miRNA 芯片技术建立大鼠腹部手术应激后血清 miRNA 差异表达谱，从中发现与早期手术创伤后应激相关的 miRNAs。方法：建立大鼠部分肝切除手术模型。检测手术前后大鼠血清中 ALT、AST 和CRP 浓度水平及肝脏病理改变，评估腹部创伤后大鼠应激情况。采用 miRNA 表达谱芯片筛选手术前后大鼠血清中差异表达的 miRNAs，并采用实时荧光定量 RT - PCR（real - time RT - PCR）进行验证。采用生物信息学软件（MiRand 和 TargetScan）预测候选 miRNA 的靶基因。结果：血清 miRNA 表达谱出现明显改变，24个miRNAs 在2/3部分肝切除术（PH）后24h 大鼠血清中表达明显升高，特别是 miR -9，其在2/3PH 术后24h 大鼠血清中的表达水平为术前的50多倍，real - time RT - PCR 检测 miR -9的结果与芯片结果相符。通过生物信息学预测，CDH1、E - cadherin、MTHFD2、PDYN、MCPIP1、BCL2L11、CMA1、Map3k1等可能是 miR -9的靶基因。结论：腹部手术创伤后，大鼠血清表达谱发生明显变化，提示 miRNA 可能参与调控创伤后应激反应。
목적：응용 miRNA 심편기술건립대서복부수술응격후혈청 miRNA 차이표체보，종중발현여조기수술창상후응격상관적 miRNAs。방법：건립대서부분간절제수술모형。검측수술전후대서혈청중 ALT、AST 화CRP 농도수평급간장병리개변，평고복부창상후대서응격정황。채용 miRNA 표체보심편사선수술전후대서혈청중차이표체적 miRNAs，병채용실시형광정량 RT - PCR（real - time RT - PCR）진행험증。채용생물신식학연건（MiRand 화 TargetScan）예측후선 miRNA 적파기인。결과：혈청 miRNA 표체보출현명현개변，24개miRNAs 재2/3부분간절제술（PH）후24h 대서혈청중표체명현승고，특별시 miR -9，기재2/3PH 술후24h 대서혈청중적표체수평위술전적50다배，real - time RT - PCR 검측 miR -9적결과여심편결과상부。통과생물신식학예측，CDH1、E - cadherin、MTHFD2、PDYN、MCPIP1、BCL2L11、CMA1、Map3k1등가능시 miR -9적파기인。결론：복부수술창상후，대서혈청표체보발생명현변화，제시 miRNA 가능삼여조공창상후응격반응。
Objective:To screen and identify the differential expression of miRNAs in rat serum post traumatic in-jury by the miRNA microarray technique. Methods:We chose partial hepatectomy(PH)as traumatic injury model. The serum ALT,AST,CRP and histological changes of the liver were measured to evaluate the severity of trauma. The miRNA microarray was used to assess the level and composition of serum miRNAs. The target genes of candidate miR-NA were predicted by bioinformatics software(MiRand and TargetScan). Results:There was significantly differential expression of miRNAs in rat serum post traumatic injury(24 miRNAs were more than 2 - fold up - regulated). Espe-cially,the expression of miR - 9 showed the highest up - regulated(more than 50 - fold). The results showed that CDH1,E - cadherin,MTHFD2,PDYN,MCPIP1,BCL2L11,CMA1,Map3k1 may be the target genes of miR - 9. Con-clusion:Injury caused by abdominal operation could make significant change in the spectra and levels of serum miR-NAs,suggesting that miRNAs may be involved in regulating the stress response post trauma.