CAJ | 학술논문

目的：利用气相色谱－质谱（GC－MS）法对采自陕西省的野生薄荷挥发油化学成分进行分析。方法采用水蒸气蒸馏法提取挥发油，取适量配制成溶液，用GC－MS进行分析。色谱柱为HP－FFAP石英毛细管柱（30 m ×0.32 mm，0.25μm），进样口温度为220℃，柱温为程序升温，初始温度为65℃，保持3 min，以5℃/min的速率升温至230℃，保持5 min，载气为N2，流速为1.5 mL/min，分流比为10：1。质谱条件，接口温度为230℃，离子源温度为220℃。结果共鉴定了5批野生薄荷挥发油中的67个化学成分，主要成分有薄荷醇、左旋香芹酮和乙酸松油酯。结论生长环境、居群和采收时间不同，陕西野生薄荷挥发油化学成分的种类和相对含量差异很大。
목적：이용기상색보－질보（GC－MS）법대채자합서성적야생박하휘발유화학성분진행분석。방법채용수증기증류법제취휘발유，취괄량배제성용액，용GC－MS진행분석。색보주위HP－FFAP석영모세관주（30 m ×0.32 mm，0.25μm），진양구온도위220℃，주온위정서승온，초시온도위65℃，보지3 min，이5℃/min적속솔승온지230℃，보지5 min，재기위N2，류속위1.5 mL/min，분류비위10：1。질보조건，접구온도위230℃，리자원온도위220℃。결과공감정료5비야생박하휘발유중적67개화학성분，주요성분유박하순、좌선향근동화을산송유지。결론생장배경、거군화채수시간불동，협서야생박하휘발유화학성분적충류화상대함량차이흔대。
Objective To analyze the chemical components of volatile oil in uncultivated Mentha arvensis L. picked from Shannxi province by GC-MS. Methods The steam distillation method was used to extract the volatile oil,then a appropriate amount of which were taken to prepare the solution for conducting analysis by GC-MS. The chromatographic column was HP-FFAP quartz capillary column(30 m × 0. 32 mm,0. 25 μm),the temperature of the injection port was 220 ℃. The column temperature was temperature pro-gramming and the initial temperature was set at 65 ℃ and kept for 3 min,then heated up to 230 ℃ with a rate of 5 ℃ /min and kept for 5 min. The carrier gas was N2 and the flow rate was 1. 5 mL/min. The split ratio was 1 :10. The mass spectrometry con-dition:the interface temperature was 230 ℃,the ion source temperature was 220 ℃. Results Totally 67 chemical compounds in the volatile oil from 5 batches of uncultivated Mentha arvensis L. were identified by GC/MS and the main components were menthol,levo-duction carvone and terpinyl acetate. Conclusion The kinds and relative contents of chemical components in volatile oil from unculti-vated Mentha arvensis L. picked from Shannxi have large difference due to different growth environments,populations and harvest time.