中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2015年
7期
34-35
,共2页
顺铂%肾损伤%血管紧张素Ⅱ%转化生长因子-β1%相关性
順鉑%腎損傷%血管緊張素Ⅱ%轉化生長因子-β1%相關性
순박%신손상%혈관긴장소Ⅱ%전화생장인자-β1%상관성
cisplatin%renal injury%angiotensinⅡ%transforming growth factor - β1%correlation
目的：观察血管紧张素Ⅱ（AngⅡ）与转化生长因子－β1(TGF －β1)在顺铂肾损伤中的作用及二者的相关性。方法取16只大鼠随机分为正常组和模型组，各8只。采用尾静脉注射的方法复制顺铂肾损伤模型，6周后以放射免疫法检测肾组织中 AngⅡ水平，免疫组化法观察 TGF －β1在肾组织中的表达。结果与正常组比较，模型组肾组织中 AngⅡ含量明显增加，TGF －β1在肾小管上皮细胞的表达明显增强（ P ﹤0.01），且二者之间有一定相关性（ Y =0.138 X ＋0.0628，r =0.98）。结论肾脏局部 AngⅡ的水平与 TGF －β1的表达可能在顺铂肾损伤的发生、发展中起一定作用，且二者呈正相关。
目的：觀察血管緊張素Ⅱ（AngⅡ）與轉化生長因子－β1(TGF －β1)在順鉑腎損傷中的作用及二者的相關性。方法取16隻大鼠隨機分為正常組和模型組，各8隻。採用尾靜脈註射的方法複製順鉑腎損傷模型，6週後以放射免疫法檢測腎組織中 AngⅡ水平，免疫組化法觀察 TGF －β1在腎組織中的錶達。結果與正常組比較，模型組腎組織中 AngⅡ含量明顯增加，TGF －β1在腎小管上皮細胞的錶達明顯增彊（ P ﹤0.01），且二者之間有一定相關性（ Y =0.138 X ＋0.0628，r =0.98）。結論腎髒跼部 AngⅡ的水平與 TGF －β1的錶達可能在順鉑腎損傷的髮生、髮展中起一定作用，且二者呈正相關。
목적：관찰혈관긴장소Ⅱ（AngⅡ）여전화생장인자－β1(TGF －β1)재순박신손상중적작용급이자적상관성。방법취16지대서수궤분위정상조화모형조，각8지。채용미정맥주사적방법복제순박신손상모형，6주후이방사면역법검측신조직중 AngⅡ수평，면역조화법관찰 TGF －β1재신조직중적표체。결과여정상조비교，모형조신조직중 AngⅡ함량명현증가，TGF －β1재신소관상피세포적표체명현증강（ P ﹤0.01），차이자지간유일정상관성（ Y =0.138 X ＋0.0628，r =0.98）。결론신장국부 AngⅡ적수평여 TGF －β1적표체가능재순박신손상적발생、발전중기일정작용，차이자정정상관。
Objective To explore the role of angiotensin (AngⅡ) and transforming growth factor - β1 (TGF - β1 ) on cisplatin induced re-nal injury and their correlation. Methods 16 rats were randomly divided into the two groups, normal group and model group. The cau-dal vein injection was adopted to duplicate the cisplatin induced renal injury model in rat. The level of AngⅡ in the renal tissue after 6 weeks was detected by radioimmunoassay and the expression of TGF - β1 in the renal tissue was observed by the immunohistochem-istry method. Results Compared with the blank group, the level of Ang Ⅱ in the model group was significantly increased and the ex-pression of TGF - β1 in the tubular epithelia was obviously enhanced( P ﹤ 0. 01), moreover certain correlation existed between them ( Y = 0. 138 X + 0. 062 8, r = 0. 98) . Conclusion The AngⅡ level and TGF - β1 expression may play a certain role in the occurrence and development of cisplatin induced renal injury and show the positive correlation.
