CAJ | 학술논문

目的：分析鼻咽癌组织中 DAPK 基因启动子的甲基化状态，探讨 DAPK 基因启动子甲基化与鼻咽癌的关系。方法：应用甲基化特异性 PCR 技术检测48例鼻咽癌组织、26例慢性鼻咽黏膜炎症组织的 DAPK 基因启动子甲基化状态，比较鼻咽癌和慢性鼻咽黏膜炎症组织的 DAPK 基因启动子甲基化率。结果：鼻咽癌组织的 DAPK 基因启动子甲基化率为75%，而慢性鼻咽黏膜炎症组织中未检测到 DAPK 基因启动子甲基化。结论：鼻咽癌组织中 DAPK 基因启动子存在高甲基化水平，检测 DAPK 基因启动子甲基化或许能为鼻咽癌的诊断提供依据。
목적：분석비인암조직중 DAPK 기인계동자적갑기화상태，탐토 DAPK 기인계동자갑기화여비인암적관계。방법：응용갑기화특이성 PCR 기술검측48례비인암조직、26례만성비인점막염증조직적 DAPK 기인계동자갑기화상태，비교비인암화만성비인점막염증조직적 DAPK 기인계동자갑기화솔。결과：비인암조직적 DAPK 기인계동자갑기화솔위75%，이만성비인점막염증조직중미검측도 DAPK 기인계동자갑기화。결론：비인암조직중 DAPK 기인계동자존재고갑기화수평，검측 DAPK 기인계동자갑기화혹허능위비인암적진단제공의거。
Objective:To analyze methylation of death - associated protein kinase gene(DAPK)promoter in na-sopharyngeal carcinoma,and to explore the relationship between carcinogenesis of nasopharyngeal carcinoma and methylation of DAPK gene promoter. Methods:Methylation specific - polymerase chain reaction assay was designed to detect methylation status of DAPK gene promoter from 48 patients with nasopharyngeal carcinoma and 26 patients with chronic rhinitis,the rate of methylation was compared for patients with nasopharyngeal carcinoma and chronic rhinitis. Results:The frequency of promoter methylation of DAPK gene was 75% in nasopharyngeal carcinoma biopsy sam-ples,while methylation was not found in chronic rhinitis biopsy samples. Conclusion:Aberrant promoter methylation of DAPK gene is found in nasopharyngeal carcinoma,and assay of methylation of DAPK gene promote may be helpful for diagnosis of nasopharyngeal carcinoma.