现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2015年
9期
1205-1209
,共5页
陈亚平%姜晓晓%冯守信%蒋冠
陳亞平%薑曉曉%馮守信%蔣冠
진아평%강효효%풍수신%장관
白介素 24%放射治疗%宫颈癌
白介素 24%放射治療%宮頸癌
백개소 24%방사치료%궁경암
IL - 24%radiation%cervical cancer
目的：观察条件复制性腺病毒（ZD55）携带白介素24（ZD55- IL -24）单独与联合放射治疗对宫颈癌Hela 细胞的治疗效果。方法：体外培养宫颈癌 Hela 细胞，分成 PBS 组、放射治疗组、ZD55- IL -24组、ZD55-IL -24联合放射治疗组。采用 MTT 法检测细胞生长抑制率，Annexin V - FITC/ PI 双染法检测细胞凋亡率，流式细胞仪检测细胞活性氧 ROS 水平，Western blot 检测凋亡蛋白 Bax、Bcl -2表达。结果：MTT 法显示 PBS 组、放射治疗组、ZD55- IL -24组、ZD55- IL -24联合放射治疗组抑制率分别为（7.28±1.99）%、（45.16±4.12）%、（33.23±2.63）%、（85.27±2.03）%，ZD55- IL -24联合放射治疗组细胞抑制率大于其他三组（P
目的：觀察條件複製性腺病毒（ZD55）攜帶白介素24（ZD55- IL -24）單獨與聯閤放射治療對宮頸癌Hela 細胞的治療效果。方法：體外培養宮頸癌 Hela 細胞，分成 PBS 組、放射治療組、ZD55- IL -24組、ZD55-IL -24聯閤放射治療組。採用 MTT 法檢測細胞生長抑製率，Annexin V - FITC/ PI 雙染法檢測細胞凋亡率，流式細胞儀檢測細胞活性氧 ROS 水平，Western blot 檢測凋亡蛋白 Bax、Bcl -2錶達。結果：MTT 法顯示 PBS 組、放射治療組、ZD55- IL -24組、ZD55- IL -24聯閤放射治療組抑製率分彆為（7.28±1.99）%、（45.16±4.12）%、（33.23±2.63）%、（85.27±2.03）%，ZD55- IL -24聯閤放射治療組細胞抑製率大于其他三組（P
목적：관찰조건복제성선병독（ZD55）휴대백개소24（ZD55- IL -24）단독여연합방사치료대궁경암Hela 세포적치료효과。방법：체외배양궁경암 Hela 세포，분성 PBS 조、방사치료조、ZD55- IL -24조、ZD55-IL -24연합방사치료조。채용 MTT 법검측세포생장억제솔，Annexin V - FITC/ PI 쌍염법검측세포조망솔，류식세포의검측세포활성양 ROS 수평，Western blot 검측조망단백 Bax、Bcl -2표체。결과：MTT 법현시 PBS 조、방사치료조、ZD55- IL -24조、ZD55- IL -24연합방사치료조억제솔분별위（7.28±1.99）%、（45.16±4.12）%、（33.23±2.63）%、（85.27±2.03）%，ZD55- IL -24연합방사치료조세포억제솔대우기타삼조（P
Objective:To evaluate the effects of the conditionally replicating adenovirus carrying interleukin - 24 combined with radiation on cervical cancer Hela cell. Methods:Hela cells were divided into four groups:PBS group, radiation group,ZD55 - IL - 24 group and ZD55 - IL - 24 with radiation group. The cell viability was assessed by MTT assay. The apoptotic rate of cells was assessed by Annexin V - FITC/ PI assay. The expression of Bax,Bcl - 2 was detected by Western blot. Results:PBS group,cell viability of radiation group,ZD55 - IL - 24 group and ZD55 -IL - 24 with radiation group were(7. 28 ± 1. 99)% ,(45. 16 ± 4. 12)% ,(33. 23 ± 2. 63 )% and(85. 27 ± 2. 03)% , which reveals cell viability of ZD55 - IL - 24 with radiation group was higher than other groups(P < 0. 05). Annexin V - FITC / PI revealed that the apoptotic rate of ZD55 - IL - 24 with radiation was higher than other groups(P< 0. 05). ROS level of ZD55 - IL - 24 with radiation was higher than other groups(P < 0. 05). The expression of Bax in ZD55 - IL - 24 with radiation group was higher than in other groups(P < 0. 05),while the expression of Bcl - 2 in ZD55 - IL - 24 with radiation group was lower than in other groups(P < 0. 05). Conclusion:The conditionally repli-cating adenovirus carrying interleukin - 24 can increase radiosensitivity of cervical cancer Hela cell.
