中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2015年
7期
55-56
,共2页
百癣夏塔热胶囊%槲皮素%反相高效液相色谱法%含量测定
百癬夏塔熱膠囊%槲皮素%反相高效液相色譜法%含量測定
백선하탑열효낭%곡피소%반상고효액상색보법%함량측정
Baixuanxiatare Capsules%quercetin%RP - HPLC%content determination
目的：建立测定百癣夏塔热胶囊中槲皮素含量的反相高效液相色谱法。方法色谱柱为 Ultimate XB － C18－ H 柱(250 mm ×4.6 mm，5μm)，流动相为甲醇－0.4%磷酸溶液(49：51)，流速为1.0 mL / min，检测波长360 nm，进样量为10μL。结果槲皮素进样量在19.50~585 ng 范围内与峰面积线性关系良好（ r =0.9999），平均加样回收率为97.69%，RSD 为0.85%( n =6)。结论该方法快速简便、准确可靠、重复性好，可用于百癣夏塔热胶囊中槲皮素的含量测定。
目的：建立測定百癬夏塔熱膠囊中槲皮素含量的反相高效液相色譜法。方法色譜柱為 Ultimate XB － C18－ H 柱(250 mm ×4.6 mm，5μm)，流動相為甲醇－0.4%燐痠溶液(49：51)，流速為1.0 mL / min，檢測波長360 nm，進樣量為10μL。結果槲皮素進樣量在19.50~585 ng 範圍內與峰麵積線性關繫良好（ r =0.9999），平均加樣迴收率為97.69%，RSD 為0.85%( n =6)。結論該方法快速簡便、準確可靠、重複性好，可用于百癬夏塔熱膠囊中槲皮素的含量測定。
목적：건립측정백선하탑열효낭중곡피소함량적반상고효액상색보법。방법색보주위 Ultimate XB － C18－ H 주(250 mm ×4.6 mm，5μm)，류동상위갑순－0.4%린산용액(49：51)，류속위1.0 mL / min，검측파장360 nm，진양량위10μL。결과곡피소진양량재19.50~585 ng 범위내여봉면적선성관계량호（ r =0.9999），평균가양회수솔위97.69%，RSD 위0.85%( n =6)。결론해방법쾌속간편、준학가고、중복성호，가용우백선하탑열효낭중곡피소적함량측정。
Objective To establish a HPLC method for deermining the quercetin content in Baixuanxiatare Capsules. Methods The sep-aration was performed on the Ultimate XB - C18 - H column with methanol - 0. 4% phosphoric acid (49: 51) as the mobile phase. The detection wavelength was 360 nm, the flow rate was 1. 0 mL / min, and the injection volume was 10 μL. Results The sample size in the range of 19. 50 - 585 ng showed a good linearity with the peak area( r = 0. 999 9 ); the average recovery rate of quercetin was 97. 69% , RSD 0. 85% ( n = 6) . Conclusion The established method is rapid, simple, accurate and reliable with good repeatability, and can be used for the determination of the quercetin content in Baixuanxiatare Capsules.
