中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2015年
7期
51-52
,共2页
血安胶囊%没食子酸%高效液相色谱法
血安膠囊%沒食子痠%高效液相色譜法
혈안효낭%몰식자산%고효액상색보법
Xuean capsules%gallic acid%HPLC
目的：建立测定血安胶囊中没食子酸含量的反相高效液相色谱法。方法色谱柱采用 Hypersil ODS2柱(200 mm ×4.6 mm，5μm)，流动相为甲醇－0.1%磷酸溶液（3：97），检测波长为214 nm，流速为1.0 mL / min。结果没食子酸进样量在0.0211~0.211μg 范围内与峰面积呈良好线性关系（ R2=1），平均回收率为99.28%，RSD =1.73%( n =6)。结论该方法简便、快速、结果准确、重复性好，可用于血安胶囊中没食子酸含量的测定。
目的：建立測定血安膠囊中沒食子痠含量的反相高效液相色譜法。方法色譜柱採用 Hypersil ODS2柱(200 mm ×4.6 mm，5μm)，流動相為甲醇－0.1%燐痠溶液（3：97），檢測波長為214 nm，流速為1.0 mL / min。結果沒食子痠進樣量在0.0211~0.211μg 範圍內與峰麵積呈良好線性關繫（ R2=1），平均迴收率為99.28%，RSD =1.73%( n =6)。結論該方法簡便、快速、結果準確、重複性好，可用于血安膠囊中沒食子痠含量的測定。
목적：건립측정혈안효낭중몰식자산함량적반상고효액상색보법。방법색보주채용 Hypersil ODS2주(200 mm ×4.6 mm，5μm)，류동상위갑순－0.1%린산용액（3：97），검측파장위214 nm，류속위1.0 mL / min。결과몰식자산진양량재0.0211~0.211μg 범위내여봉면적정량호선성관계（ R2=1），평균회수솔위99.28%，RSD =1.73%( n =6)。결론해방법간편、쾌속、결과준학、중복성호，가용우혈안효낭중몰식자산함량적측정。
Objective To establish a RT - HPLC method for determining the gallic acid content in Xuean Capsule by RP - HPLC. Methods The octadecyl silane bonded silica gel column ODS - C18 was adopted with the mixture solvent of methanol - 0. 1% phospho-ric acid (3 : 97) as the mobile phase, the flow rate was 1. 0 mL / min and the detecting wavelength was 214 nm. Results The sample size of gallic acid in the range of 0. 021 1 - 0. 211 μg / mL showed the good linear relation with the peak area. The average recovery rate was 99. 28% , RSD = 1. 73% ( n = 6 ) . Conclusion It is concluded that with the parameter set used in this study, the gallic acid content in Xuean capsules can be determined in a fast and accurate manner with a good reproducibility.
