CAJ | 학술논문

目的：研究通心络对自发性高血压大鼠（ SHR ）肾损害的干预作用及机制。方法10周龄雄性SHR 48只随机分成5组：SHR对照组（同龄Wistar大鼠作对照），氨氯地平干预组和氨氯地平＋不同剂量通心络（0.5，1.0，1.5 g· kg－1· d－1）干预组。20周后，测血压、留尿后处死，取肾组织检测指标。测尿微量白蛋白（ UMA）和β2微球蛋白（β2 MG），测血管紧张素转化酶2、血管紧张素Ⅱ1型受体基因表达。结果与同龄京都威斯特（ WKY）大鼠比较，10周龄SHR的尿β2 MG、肾组织AT1 R表达明显增加而ACE2明显减少。与单用氨氯地平组比较，伍用通心络组大鼠的尿β2 MG、UMA及肾组织AT1 R表达明显减少而肾组织ACE2表达明显增加。结论氨氯地平伍用通心络能降低蛋白尿、减轻高血压病肾损害的作用可能是通过增加肾组织ACE2、下调AT1 R表达来实现的。
목적：연구통심락대자발성고혈압대서（ SHR ）신손해적간예작용급궤제。방법10주령웅성SHR 48지수궤분성5조：SHR대조조（동령Wistar대서작대조），안록지평간예조화안록지평＋불동제량통심락（0.5，1.0，1.5 g· kg－1· d－1）간예조。20주후，측혈압、류뇨후처사，취신조직검측지표。측뇨미량백단백（ UMA）화β2미구단백（β2 MG），측혈관긴장소전화매2、혈관긴장소Ⅱ1형수체기인표체。결과여동령경도위사특（ WKY）대서비교，10주령SHR적뇨β2 MG、신조직AT1 R표체명현증가이ACE2명현감소。여단용안록지평조비교，오용통심락조대서적뇨β2 MG、UMA급신조직AT1 R표체명현감소이신조직ACE2표체명현증가。결론안록지평오용통심락능강저단백뇨、감경고혈압병신손해적작용가능시통과증가신조직ACE2、하조AT1 R표체래실현적。
Objective To explore the effect of Tong Xin Luo ( TXL) on the renal damage by hypertension in spontaneously hypertensive rats ( SHR).Methods Ten week -old male SHR ( n=48 ) were randomly divided into 5 groups:control group , the amlodipine group , the amlodi-pine and different dose TXL groups.The same old male Wistor -Kyoto ( WK) rats was as control group .At the time of 20 weeks later after treatment, blood pressure, urine microalbumin, urine β2 -microglobulin were detected , the gene expression of angiotensin -converting enzyme 2 (ACE2) and angiotensin II type 1 receptor (AT1R) in rats kidney were determined.Results Compared with Wistar -Kyoto ( WKY ) control group, 10 week -old SHR showed higher levels of urine β2 MG and ACE2 , but less AT1 R.After treatment for 20 weeks, compared with the amlodipine group , amlodipine with TXL reduced urinary β2 -microglo-bulin and urine microalbumin , decreased AT 1 R expression and increased ACE2 expression.Conclusion Taking high-dose TXL with antihyper-tensive therapy could prevent renal damage progression , and its effect may be through lowering albuminuria , increasing renal ACE 2 expression , and decreasing AT 1 R expression.